Team:NTNU Trondheim/Notebook

From 2012.igem.org

(Difference between revisions)
(Thursday 09.08.12)
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Did a restriction digest as described in the protocol on the following parts.
+
Did a restriction digest as described in the [http://2012.igem.org/Team:NTNU_Trondheim/Protocols protocol] on the following parts. On the pieces to be used as inserts, the number of base pairs are also listed.
{| class="wikitable" style="text-align:center;margin: 1em auto 1em auto;"
{| class="wikitable" style="text-align:center;margin: 1em auto 1em auto;"
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!Enzymes
!Enzymes
!Buffer
!Buffer
 +
!bp insert
|-
|-
|LuxI+term
|LuxI+term
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|XbaI+PstI
|XbaI+PstI
|3
|3
 +
|747
 +
|-
 +
|YFP+term
 +
|<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>
 +
|XbaI+PstI
 +
|3
 +
|852
|-
|-
|Lysis
|Lysis
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|XbaI+PstI
|XbaI+PstI
|3
|3
 +
|1785
|-
|-
|LuxI
|LuxI
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|XbaI+PstI
|XbaI+PstI
|3
|3
 +
|618
|-
|-
|RBS*
|RBS*
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|SpeI+PstI
|SpeI+PstI
|1
|1
 +
| -
|-
|-
|P<sub>luxR+HSL</sub>
|P<sub>luxR+HSL</sub>
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|SpeI+PstI
|SpeI+PstI
|1
|1
 +
| -
|-
|-
|RBS
|RBS
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|SpeI+PstI
|SpeI+PstI
|1
|1
 +
| -
|-
|-
|pSB1A3
|pSB1A3
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|EcoRI+PstI+DpnI
|EcoRI+PstI+DpnI
|3
|3
 +
| -
|-
|-
|plld
|plld
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|EcoRI+SpeI
|EcoRI+SpeI
|4
|4
 +
|433
|-
|-
|}
|}
-
The parts lysis, YFP+term, pSB1A3 and plld will be assembled using the 3A assembly, made double cutting mix of these.
+
The parts lysis <partinfo>BBa_K112808</partinfo>, YFP+term (<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>), <partinfo>pSB1A3</partinfo> and plld will be assembled using the 3A assembly, so made double cutting mix with these parts.
 +
 
 +
Gel electrophoresis was done with luxI+term (<partinfo>Bba_C0061</partinfo>, <partinfo>BBa_B0015</partinfo>), lysis <partinfo>BBa_K112808</partinfo>, YFP+term (<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>), LuxI <partinfo>Bba_C0061</partinfo> and plld.
===Wednesday 08.08.12===
===Wednesday 08.08.12===

Revision as of 12:31, 9 August 2012

NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze


Notebook

February
Mon Tue Wed Thu Fri Sat Sun
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 25
27 28 29
March
Mon Tue Wed Thu Fri Sat Sun
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
April
Mon Tue Wed Thu Fri Sat Sun
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
May
Mon Tue Wed Thu Fri Sat Sun
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
June
Mon Tue Wed Thu Fri Sat Sun
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
July
Mon Tue Wed Thu Fri Sat Sun
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
August
Mon Tue Wed Thu Fri Sat Sun
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
September
Mon Tue Wed Thu Fri Sat Sun
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

Click on a month name or date to view the respective notebook entries. For information on plasmids and DNA mentioned in the notebook, see Stocks and Constructs. For explanations of abbreviations used, click here.

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Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Notebook"