Team:NTNU Trondheim/Logg


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Thursday 03.05.12

We had a meeting, and we discussed several things we would like to look more into before we start planning what our genetic circuit will actually look like.

Here is a list of what we decided to do, and who will do it:

  • Check Christopher Anderson's article to see how similar his project was to ours - Eirin
  • Make a calendar on our wiki page - Ove
  • Look deeper into what kind of anti cancer drugs we could make our cells synthetize - Nina
  • Check if we have access to cancer cells, and find out more about molecules that are secreted form cancer cells in larger amounts than by normal cells - Gunvor
  • Find out more about the O2-sensitive promoter - Rahmi
  • Rolf was also goint to do something, but I have forgotten what he was going to do...

- We also decided to have our next meeting at 13:30 next friday, and we decided to eat lunch together on wednesdays

- Rahmi came with a suggestion for characterization of the O2-sensitive promoter:

We could make the promoter control the expression of a fluorescent protein, then transform the system to cells, grow the cells on a soft medium they could migrate into, let them grow for a while, and then investigate the medium for coloured cells.
Suggestion from Gunvor: We could use TIRF (Total Internal Reflection Fluorescence) microscope, which allows us to investigate the fluorescence in a certain layer of the medium.