Team:NTNU Trondheim/Constructs

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NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze
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Test and working constructs
Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
pllD pllD promoter in pSB1C3pSB1C3HighTransformed but unverified
VGB+RBS+YFP+DTTBBa_K561001, BBa_B0034, BBa_E0030,BBa_B0015VGB test construct??Transformed. Untested.
VHB+RBS+YFP+DTTBBa_K258005, BBa_B0034, BBa_E0030, BBa_B0015VHB test construct??Transformed. Untested.
K+RBS+RFP+DTTBBa_K081005,BBa_E1010,BBa_B0015RFP test construct??Negative results
K+RBS+YFP+DTTBBa_K081005,BBa_E0030,BBa_B0015YFP test construct??Works
K+RBS+GFP+DTTBBa_K081005,BBa_K082003,BBa_B0015GFP test construct??Negative results
PBAD+lysis BBa_K206000, BBa_K112808Arabinoseindusert promotor med lysis device pSB1A3 Testet og fungerer
Intermediates (constructs that have no function alone)
Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
DLD+RBS BBa_K284003, BBa_B0034Laktatpromotor med RBSpSB1A2 (2079) High Testkuttet
JEN1+RBS BBa_K284003, BBa_B0034Laktatpromotor med RBSpSB1A2 (2079) High Testkuttet
VGB+RBS BBa_K284003, BBa_B0034Oksygenpromotor med RBS pSB1A2 (2079) High Testkuttet
VHB+RBS BBa_K284003, BBa_B0034Oksygenpromotor med RBS pSB1A2 (2079) High Testkuttet
RFP+DTT BBa_E1010, BBa_B0015Fluorescerende protein med terminatorpSB1AK3 (3189) Testkuttet
YFP+DTT BBa_E0030, BBa_B0015Fluorescerende protein med terminatorpSB1AK3 (3189) Testkuttet
CFP+DTT BBa_E0020, BBa_B0015Fluorescerende protein med terminatorpSB1AK3 (3189) Testkuttet
GFP(+LVA)+DTT BBa_K082003, BBa_B0015Fluorescerende protein med terminatorpSB1AK3 Testkuttet
LuxR+DTT BBa_C0062, BBa_B0015LuxR-gen med terminatorpSB1AK3 Ligert og transformert
LuxI+DTT BBa_C0061, BBa_B0015LuxI-gen med terminatorpSB1AK3 Ligert og transformert
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