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. We have BioBricks! | . We have BioBricks! | ||
| - | . Practice run of growth study | + | . Practice run of growth study involving the characterisation |
==Day 2== | ==Day 2== | ||
Welcome to the NRP UEA iGEM 2012 Wiki Lab Book
Please choose the relevant link to access our diary of that week!
Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments
Contents |
. We have BioBricks!
. Practice run of growth study involving the characterisation
. LB media and Agar media was made in vast quantities.
. Rebecca did a 9 hour growth study with PyeaR transfoormed cells and Bioline alpha E.coli cells as a comparison. This was done with wavescan function and single wavelength absorbance at 660nm which is not absorbed by pure LB. Recordings were taken every hour and a serial dilution was made and the two lowest dilutions were plated (10^-4 and 10^-5).
. Forum preparation - plates with Biobrick written with one letter per plate (using the pYEAR with GFP and potassium nitrate to induce promoter activity)
. Rebecca, Khadija and Lucas, used day 2 samples and carried out a growth study with a reading every minute for 1 hour.
- Forum preparation - plates used for the forum science day had their fluorescence intensified by adding more potassium nitrate to the cells on the plate.
. UK Team Meet-Up!
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