Team:NRP-UEA-Norwich/Week5

From 2012.igem.org

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==Day 2==
==Day 2==
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. Rachel and Russell calculated the expected length of plasmid and found that the plasmids on the agarose gel were completely different to expected. Decided to carry out a restriction digest of the plasmids to remove the inserts and then run those on a gel to find out if they are as expected.
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. Rachel and Russell calculated the expected length of plasmid and found that the plasmids on the agarose gel were completely different to expected. Decided to carry out a restriction digest of the plasmids to remove the inserts and then run those on a gel to find out if they are as expected. After a nanodrop we found that there was a very small amount of DNA and likely too little to work with; mini-prepped the second samples for each biobrick and nanodropped them.
==Day 3==
==Day 3==

Revision as of 11:34, 7 August 2012

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NRP UEA iGEM 2012

 

Welcome to the NRP UEA iGEM 2012 Wiki Lab Book

Please choose the relevant link to access our diary of that week!

Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments

Contents

Week 5

Day 1

. Rachel and Russell ran their RFP/eCFP/AraC isolated plasmids on an agarose gel to see if they were there. The results of the gel seemed unusual.

Day 2

. Rachel and Russell calculated the expected length of plasmid and found that the plasmids on the agarose gel were completely different to expected. Decided to carry out a restriction digest of the plasmids to remove the inserts and then run those on a gel to find out if they are as expected. After a nanodrop we found that there was a very small amount of DNA and likely too little to work with; mini-prepped the second samples for each biobrick and nanodropped them.

Day 3

Day 4

Day 5

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