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| - | [[File:NRPNOLogo.png | centre]] | + | [[File:NRPNOLogo.png | centre | https://2012.igem.org/Team:NRP-UEA-Norwich/NOSensing]] |
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Please choose the relevant link to view an overview of each project!
Nitric Oxide Sensing & The Hybrid Promoters | The Comparator Circuit | Theoretical Projects
Sensory BioBrick systems have been a large constituent of previous iGEM projects, in which teams have combined impressive amounts of logic with limitless creativity to produce synthetically engineered organisms with the potential of detecting the presence of a substrate within its environment via innovative combinations of various promoter and reporter BioBricks. Our project comprised of 3 novel systems aims to revolutionise the way sensory systems work.
Our own hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably.
. Flexible and has two orientations
. Six biobricks out of it
. Characterised with fluorescent proteins
. Data from flow cytometry, fluorometers, FACS
. Transfected into mammalian cells for further testing
The lack of specificity of the bacterial promoter, pYEAR, used in the hybrid promoter was a pitfall that was always a concern. From this potential problem spawned a potential solution; the Comparator Circuit. This pair of BioBricks are designed to specifically bind to each other while ligated to promoters of overlapping specificity to result in an integrating of the conflicting outputs of the two opposing gene systems.
In essence, what we have created is a pair of antagonistic BioBricks that turned the pair of mRNAs in which they reside into translational repressor molecules when both are transcribed in tandum within a specific chassis of interest.
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