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Team:NRP-UEA-Norwich - Revision history
2024-03-28T15:12:08Z
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RussellGritton at 00:57, 27 September 2012
2012-09-27T00:57:37Z
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RussellGritton
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RussellGritton at 00:57, 27 September 2012
2012-09-27T00:57:11Z
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RussellGritton
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RussellGritton at 12:59, 26 September 2012
2012-09-26T12:59:44Z
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RussellGritton
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Joyehicks at 09:40, 26 September 2012
2012-09-26T09:40:41Z
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Joyehicks
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RussellGritton at 15:14, 25 September 2012
2012-09-25T15:14:02Z
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RussellGritton
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RussellGritton at 21:55, 16 September 2012
2012-09-16T21:55:12Z
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RussellGritton
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RussellGritton at 21:54, 16 September 2012
2012-09-16T21:54:51Z
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RussellGritton
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RussellGritton at 14:10, 3 September 2012
2012-09-03T14:10:06Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">= '''[https://2012.igem.org/Team:NRP-UEA-Norwich/Project Our project] - at a glance''' =</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The team were initially interested in the role of nitric oxide (NO) in biological systems and were aiming to produce a BioBrick that can sense NO within an environment, report on the levels, and go on to make relevant and desirable changes. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">While researching possible promoters that can be used to detect NO, we decided to hybridise two promoter elements; PyeaR, a bacterial promoter, and the NS2E9 element, found in mammals. Both promoters, when on their own, use NO as an inducer molecule. The team aim to use a chassis of ''Escherichia coli'' in order to characterise the hybridised promoter, before investigating its competency within a mammalian cell chassis (MCF7, Michigan Cancer Foundation cell line variant 7, cells) for future applications within a medical system. We also aim to characterise various promoters produced by previous iGEM teams, especially those involved with sensing physiologically-relevant molecules and reporting with fluorescence or bioluminescence.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The team then turned their attention to developing the comparator circuit, a new system they feel will revolutionise transcriptional regulation via the formation of mRNA duplexes that sequester the translation of any gene after the comparator circuit biobrick. We hope, using "Escherichia coli" and a number of standard reporter biobricks, to show the functionality of this protein knock-out system and also introduce it to the registry.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Possible applications we see for both projects include; cancer therapeutics, where NO levels are detected and raised above the level at which it aids angiogenesis and into the levels at which it is toxic to a tumour; and environmental aspects, where the bio-sensor may be attached to bioluminescence in order to report levels of NO in soil for instance. </del></div></td><td colspan="2"> </td></tr>
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RussellGritton
http://2012.igem.org/wiki/index.php?title=Team:NRP-UEA-Norwich&diff=93244&oldid=prev
RussellGritton at 14:06, 3 September 2012
2012-09-03T14:06:35Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{UEANRP}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{UEANRP}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font face=arial size=6pt color=blue><a href=https://2012.igem.org/Team:NRP-UEA-Norwich/Project><b>Our Project</a></font><font size=6pt> - at a glance</font></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font face=arial size=6pt color=blue><a href=https://2012.igem.org/Team:NRP-UEA-Norwich/Project><b>Our Project</a></font><font size=6pt> - at a glance</font></b></div></td></tr>
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RussellGritton
http://2012.igem.org/wiki/index.php?title=Team:NRP-UEA-Norwich&diff=93243&oldid=prev
RussellGritton at 14:06, 3 September 2012
2012-09-03T14:06:03Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> rpp: 15,</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> rpp: 15,</div></td></tr>
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RussellGritton