"
Team:Missouri Miners/Project
From 2012.igem.org
(Prototype team page) |
(→Overall project) |
||
| Line 35: | Line 35: | ||
== '''Overall project''' == | == '''Overall project''' == | ||
| - | + | Tuberculosis is caused by a Mycobacteria tuberculosis infection that can arise from ingesting or inhaling M. tuberculosis tubercle bacilli. The bacteria then take residence within the body, mainly the lungs, causing painful lesions and possibly death. The body’s natural defense to M. tuberculosis infections is to ingest the cells by means of endocytes, but all Mycobacterium produce a waxy coating made up of free mycolic fatty acids and the endocytes cannot breakdown the bacteria. I propose to use synthetic biology to create a hybrid protein of the Clostridium cellulovorans’ cellulosome and the peroxisomal multi -functional protein 2 to be able to breakdown extracellular free fatty acids that will allow anti – TB drugs and endocytes to stop the growth or kill the infectious bacteria. This system will implemented into E. coli and further characterization and analysis will be done with those cells. | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
== Project Details== | == Project Details== | ||
Revision as of 23:51, 20 April 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Overall project
Tuberculosis is caused by a Mycobacteria tuberculosis infection that can arise from ingesting or inhaling M. tuberculosis tubercle bacilli. The bacteria then take residence within the body, mainly the lungs, causing painful lesions and possibly death. The body’s natural defense to M. tuberculosis infections is to ingest the cells by means of endocytes, but all Mycobacterium produce a waxy coating made up of free mycolic fatty acids and the endocytes cannot breakdown the bacteria. I propose to use synthetic biology to create a hybrid protein of the Clostridium cellulovorans’ cellulosome and the peroxisomal multi -functional protein 2 to be able to breakdown extracellular free fatty acids that will allow anti – TB drugs and endocytes to stop the growth or kill the infectious bacteria. This system will implemented into E. coli and further characterization and analysis will be done with those cells.
