Team:Missouri Miners/OfficialPageRemindMeToChangeThisLink

From 2012.igem.org

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<h1>BBa_K822000: MiniA</h1>
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<h1>Mini CipA coding region</h1>
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<p>This part codes for a portion of the scaffoldin protein (cipA) from Clostridium thermocellum cellulosome. Two regions of cipA were ligated together to form this part: three type one cohesin regions and a type two dockerin region. A signaling peptide for export of the protein out of the cell is also coded in this part.
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<p>Miniaturized scaffoldin protein from <i>Clostridium thermocellum</i> contains three type 1 cohesin regions and had the cellulose binding region removed from the DNA sequence along with six other cohesin regions by PCR amplification</p>
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This part is unfinished; it contains 6 pst1 sites within its coding sequence.</p>

Revision as of 04:10, 30 September 2012

Mini CipA coding region

This part codes for a portion of the scaffoldin protein (cipA) from Clostridium thermocellum cellulosome. Two regions of cipA were ligated together to form this part: three type one cohesin regions and a type two dockerin region. A signaling peptide for export of the protein out of the cell is also coded in this part. This part is unfinished; it contains 6 pst1 sites within its coding sequence.