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Team:Michigan/Project
From 2012.igem.org
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<p class="title">Notebooks</p> | <p class="title">Notebooks</p> | ||
| - | <a href="/Team:Michigan/Notebook"><span class="text-image">HbiF Standardization</span></a> | + | <a href="/Team:Michigan/Notebook/Synthesis"><span class="text-image">HbiF Standardization</span></a> |
<p><br/></p> | <p><br/></p> | ||
<a href="/Team:Michigan/Notebook"><span class="text-image">Modeling</span></a> | <a href="/Team:Michigan/Notebook"><span class="text-image">Modeling</span></a> | ||
Revision as of 05:13, 6 July 2012
Abstract
Recombinases can be used to create responsive genetic elements in biological systems. Further, it is theoretically possible to create complex control circuits using combinations of invertible DNA sequences. We utilized HbiF to augment an existing recombinase system in Escherichia coli that relied on FimE. A burst of induced, low level expression of one recombinase is expected to invert the promoter flanked by the recombinase binding sites IRR and IRL, triggering a switch from strong expression of one to another set of proteins made downstream. Induced expression of the second recombinase is expected to revert the promoter to its original orientation, triggering the original set of protein expression. The inversion will be sustained across cell divisions with little leaky protein expression and negligible performance degradation after repeated inversions. This is a heritable, binary memory system and can be used as a component in more complex systems.
Modeling
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Recombinase Characterization
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.
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