Team:Michigan/Notebook

From 2012.igem.org

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[[Team:Michigan/Notebook/Protocols]]
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!align="center"|[[Team:Michigan/Attributions|Attributions]]
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    <p><a href="/Team:Michigan/Project">Project</a> > <a href="/Team:Michigan/Noteboook">Notebook</a></p>
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<h2>Modify HbiF into a Biobrick compatible format </h2>
 
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<p>HbiF and LVA degradation tag were ordered from IDT with the following specifications.</p>
 
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">EcoRI</span></p>
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<p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p>
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<p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p>
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">AgeI</span></p>
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<p> <span style="background-color:#E0771B;font-family:Courier, monospace;">NgoMIV</span></p>
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<p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :<br/></p>
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<h2>Cloning Team Notebook</h2>
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<iframe width=100% height=800; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&amp;embedded=true"></iframe>
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<p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p>
 
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<h2>Prepare FimE for protein expression</h2>
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<iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&amp;embedded=true"></iframe>
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<h2>Team Reports</h2>
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<iframe src="https://docs.google.com/presentation/embed?id=17czITWHCoyzbzdYCvXWmwpDbl31jJyhgNmb32IXX3aY&start=false&loop=false&delayms=8000" frameborder="0" width=60% height=400 allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe>
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Latest revision as of 02:36, 4 October 2012

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