Team:Macquarie Australia/Protocols/ligations

From 2012.igem.org

Revision as of 00:24, 21 September 2012 by Ryankenny (Talk | contribs)



Ligation Procedure

A reaction mixture was prepared containing,

Element Volume
Upstream Digestion part 2 µL
Downstream Digestion part 2 µL
Destination Plasmid 2 µL
10X T4 DNA ligase buffer 2 µL
T4 DNA ligase 1 µL
H2O 11 µL