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Team:Macquarie Australia/Protocols/ MediaPrep
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| - | + | ==='''Preparation'''=== | |
| - | + | ||
| - | Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle). | + | |
| + | *'''LB media:''' (1L total) | ||
| + | **'''Ingredients:''' Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml. | ||
| + | **'''Methods:''' Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle). | ||
Revision as of 01:15, 4 September 2012
Preparation
- LB media: (1L total)
- Ingredients: Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml.
- Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle).
