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Team:Macquarie Australia/Protocols/GibsonTips
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| - | <br>The whole technique revolves around the overlapping sequence, <b>everything</b> can be planned but if this is not correct then the blocks won't anneal. Therefore, the overlapping regions need to be identified and conserved between the two adjacent sections G Blocks.</li> | + | <br>The whole technique revolves around the overlapping sequence, <b>everything</b> can be planned but if this is not correct then the blocks won't anneal. Therefore, the overlapping regions need to be identified and conserved between the two adjacent sections G Blocks. Keep an eye on the Melting Temperature (T<sub>M</sub>), of this gets to high then significant problems will arise.</li> |
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Revision as of 10:17, 20 September 2012
Top Ten Tips for Gibson Assembly
- Consider the overlapping sequence
The whole technique revolves around the overlapping sequence, everything can be planned but if this is not correct then the blocks won't anneal. Therefore, the overlapping regions need to be identified and conserved between the two adjacent sections G Blocks. Keep an eye on the Melting Temperature (TM), of this gets to high then significant problems will arise.
