Team:Macquarie Australia/Protocols/ArrivalofGBlocks

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Revision as of 08:42, 31 August 2012 by Ehoyer (Talk | contribs)



Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:

Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.


BioBrick name Fragment(s)
T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E


Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. The designed sequences of the 5 fragments can be viewed here

BioBrick name Fragment(s)
Deinococcus radiodurans bacteriophytochrome Deino_A + Deino_B + Deino_C + Deino_D + Deino_E + Plasmid