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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
(Difference between revisions)
| Line 24: | Line 24: | ||
| Hemo_A + Hemo_B + Plasmid | | Hemo_A + Hemo_B + Plasmid | ||
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| - | ! scope="row"| T7 | + | ! scope="row"| T7 Agrobacterium |
| Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid | | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid | ||
|- | |- | ||
Revision as of 08:28, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
| BioBrick name | Fragment(s) |
|---|---|
| T7 Heme oxygenase | Hemo_T7_A + Hemo_B + Plasmid |
| Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid |
| T7 Agrobacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
| Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E |
