Team:Macquarie Australia/Protocols/ArrivalofGBlocks

From 2012.igem.org

(Difference between revisions)
Line 49: Line 49:
! Agro_D
! Agro_D
! Agro_E
! Agro_E
 +
 +
{| class="wikitable"
 +
|-
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! scope="col"| BioBrick name
 +
! scope="col"| Fragment(s)
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|-
 +
! scope="row"| T7 Heme oxygenase
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| Hemo_T7_A + Hemo_B + Plasmid
 +
|-
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! scope="row"| Heme oxygenase (T7 minus)
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| Hemo_A + Hemo_B + Plasmid
 +
 +
! scope="row"| T7 Agro bacterium
 +
| Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
 +
|-
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! scope="row"| Agrobacterium (T7 minus)
 +
| Agro_A + Agro_B + Agro_C + Agro_D + Agro_E
 +
 +
|}

Revision as of 08:26, 31 August 2012



Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:

Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.


Biobrick Name Part(s) for BioBrick
T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B
Heme oxygenase (T7 minus) Plasmid Hemo_A Hemo_B
T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E
Agro (T7 minus) Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E
BioBrick name Fragment(s)
T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid T7 Agro bacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E
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