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Team:Macquarie Australia/Notebook
From 2012.igem.org
Contents |
Tuesday 7/8/12
(Click on headings to visit methods)
Making liquid media, plates & buffers
- Liquid LB Media
- SOC Solution
- SOB Solution
- LB Agar Plates
Ampicillin LB Agar Plates: 31 plates
Chloramphenicol LB Agar Plates: 33 Plates
Kanamyacin LB Agar Plates: 32 Plates
- TB buffer.
- TAE buffer.
- EDTA buffer.
Designing Gibson Assembly Fragments
- Haemoxygenase Gene
- Deinococcus radiodurans Bacteriophytochrome Gene
- Agrobacterium tumefaciens Bacteriophytochrome Gene
Tuesday 14/8/12
(Click on headings to visit methods)
Making Competent Cells
- Choosing Biobricks
- Making competent cells
- Transformations
Outreach Planning Labwork
- Open Day
- Secondary Education Seminars
Wednesday 15/8/12
Our open day preparation did not go as planned. Most of our transformations were unsuccessful, we believe this was because SOC was added after the heat shock. As disappointing as this was, there was one little piece of good news.
A single colony of Top 10 E.coli containing GFP insert was identified on a plate of LB agar media.
LB agar and nutrient agar plates were struck using this colony. The colony was also used to inoculate 5mL of nutrient broth. Plates and stock were then incubated at 37°C overnight. Both of the streaked plates and the broth showed significant growth of E.coli colonies which could fluoresce when exposed to UV light.
Unfortunately, Qantas declined to sponsor the effort of getting our team to Hong Kong. This was soon forgotten when the Macquarie Faculty of Medicine, Agilent and the Defence Science Institute (thanks Yagiz) both agreed to sponsor our team. Hong Kong here we come.
Tuesday 21/8/12
Outreach Planning Labwork Part Two
- Glowing fruit
- Secondary education seminars
- Reattempting glowing bacteria
