http://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&feed=atom&action=historyTeam:MIT/ResultsOverview - Revision history2024-03-28T21:26:35ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=301485&oldid=prevNhall at 02:34, 28 September 20132013-09-28T02:34:28Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">2012 </ins>MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td></tr>
</table>Nhallhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=297438&oldid=prevKbodner at 03:25, 27 October 20122012-10-27T03:25:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li> physically submitted 22 of our best <a href="https://2012.igem.org/Team:MIT/ResultsBiobricks">standard parts</a> to the Registry of Biological Parts </ins></div></td></tr>
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</table>Kbodnerhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=296315&oldid=prevRlearsch at 02:41, 27 October 20122012-10-27T02:41:23Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/NOTGate#NOTgate_modelbio">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">inverted mRNA sensor</a>. <del class="diffchange diffchange-inline">Check it out!</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/NOTGate#NOTgate_modelbio">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">inverted mRNA sensor</a>.</div></td></tr>
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</table>Rlearschhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=296307&oldid=prevRlearsch at 02:40, 27 October 20122012-10-27T02:40:58Z<p></p>
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</table>Rlearschhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=296288&oldid=prevRlearsch at 02:39, 27 October 20122012-10-27T02:39:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> established the <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction">viability of RNA</a> as an alternative processing medium to DNA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> established the <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction">viability of RNA</a> as an alternative processing medium to DNA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> demonstrated RNA strand displacement <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#exciting"><i>in vivo</i></a> and <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#SDbio"><i>in vitro</i></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> demonstrated RNA strand displacement <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#exciting"><i>in vivo</i></a> and <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#SDbio"><i>in vitro</i></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <del class="diffchange diffchange-inline">successfully </del><a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#NADbio">delivered DNA and RNA</a> into mammalian cells</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#NADbio">delivered DNA and RNA</a> into mammalian cells <ins class="diffchange diffchange-inline">successfully </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/NOTGate#m2bio">DNA-based NOT gate</a>, compatible with strand displacement circuits</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/NOTGate#m2bio">DNA-based NOT gate</a>, compatible with strand displacement circuits</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/Sensing">modular mRNA sensor</a> along with an inverting sensor, both compatible with strand displacement circuits</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/Sensing">modular mRNA sensor</a> along with an inverting sensor, both compatible with strand displacement circuits</div></td></tr>
</table>Rlearschhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=296036&oldid=prevCsvoss at 02:24, 27 October 20122012-10-27T02:24:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/NOTGate#<del class="diffchange diffchange-inline">model</del>">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">inverted mRNA sensor</a>. Check it out!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/NOTGate#<ins class="diffchange diffchange-inline">NOTgate_modelbio</ins>">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">inverted mRNA sensor</a>. Check it out!</div></td></tr>
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</table>Csvosshttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=294455&oldid=prevFelixsun at 01:24, 27 October 20122012-10-27T01:24:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> established the <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsFoundational</del>">viability of RNA</a> as an alternative processing medium to DNA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> established the <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">TheKeyReaction</ins>">viability of RNA</a> as an alternative processing medium to DNA</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> demonstrated RNA strand displacement <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsFoundational</del>#exciting"><i>in vivo</i></a> and <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsFoundational</del>#SDbio"><i>in vitro</i></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> demonstrated RNA strand displacement <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">TheKeyReaction</ins>#exciting"><i>in vivo</i></a> and <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">TheKeyReaction</ins>#SDbio"><i>in vitro</i></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> successfully <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsFoundational</del>#NADbio">delivered DNA and RNA</a> into mammalian cells</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> successfully <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">TheKeyReaction</ins>#NADbio">delivered DNA and RNA</a> into mammalian cells</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsProcessing</del>#m2bio">DNA-based NOT gate</a>, compatible with strand displacement circuits</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">NOTGate</ins>#m2bio">DNA-based NOT gate</a>, compatible with strand displacement circuits</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsSensing</del>">modular mRNA sensor</a> along with an inverting sensor, both compatible with strand displacement circuits</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">Sensing</ins>">modular mRNA sensor</a> along with an inverting sensor, both compatible with strand displacement circuits</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested various forms of regulating gene expression at the RNA level, compatible with strand displacement circuits, using:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> designed, modeled, and tested various forms of regulating gene expression at the RNA level, compatible with strand displacement circuits, using:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsActuation</del>#m6bio">miRNA-induced RNAi knockdown</a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">Actuation</ins>#m6bio">miRNA-induced RNAi knockdown</a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsActuation</del>#m40bio">Decoy & TuD</a> mediated double-repression systems</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">Actuation</ins>#m40bio">Decoy & TuD</a> mediated double-repression systems</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> self-cleaving <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsProcessing</del>#<del class="diffchange diffchange-inline">m3bio</del>">Hammerhead ribozymes</a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> self-cleaving <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">CircuitProduction</ins>#<ins class="diffchange diffchange-inline">HHs_productionbio</ins>">Hammerhead ribozymes</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsProcessing</del>#model">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsSensing</del>#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/<del class="diffchange diffchange-inline">ResultsSensing</del>#sensing2bio">inverted mRNA sensor</a>. Check it out!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">NOTGate</ins>#model">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">Sensing</ins>#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/<ins class="diffchange diffchange-inline">Sensing</ins>#sensing2bio">inverted mRNA sensor</a>. Check it out!</div></td></tr>
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</table>Felixsunhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=292021&oldid=prevFelixsun at 23:31, 26 October 20122012-10-26T23:31:15Z<p></p>
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</table>Felixsunhttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=268075&oldid=prevCsvoss at 03:58, 4 October 20122012-10-04T03:58:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Over the past few months we have:</div></td></tr>
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</table>Csvosshttp://2012.igem.org/wiki/index.php?title=Team:MIT/ResultsOverview&diff=268049&oldid=prevCsvoss at 03:57, 4 October 20122012-10-04T03:57:54Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</del></div></td><td colspan="2"> </td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.</ins></div></td></tr>
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</table>Csvoss