Team:MIT/ResultsBiobricks

From 2012.igem.org

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<div class= "section" id="b1bio">
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<h1>Our Favorite BioBricks</h1>
<h1>Our Favorite BioBricks</h1>
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<h2>BBa_K779501: Long Reporter top strand</h2>
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<TABLE width='700px'><TR><TD width='50%'><B>Favorite MIT 2012 iGEM Team Parts</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'><A class='noul_link' HREF='http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=MIT&Editing=Editing'><I>Edit</I></A></TABLE></form><TABLE class='pgrouptable addborder' style='width: 925px;' cellpadding='0' cellspacing='0'><TR><TH colspan='2' style='width: 1em'  nowrap><A style='color:blue;text-decoration:none;'
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<p>
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            href='http://partsregistry.org/wiki/index.php/Help:Availability_and _usefulness'>-?-</A><TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length<TH class='blank_col'>
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<a href="http://partsregistry.org/Part:BBa_K779501">Registry entry</a>
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One half of our reporter RNA complex.  When this RNA strand is displaced from its bottom strand, the RQ fluorescence quencher on this strand separates from a ROX fluorescent molecule on the bottom strand, causing the bottom strand to fluoresce.  We arrived at this design after numerous iterations over the course of the summer.
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Characterization on <a href="http://2012.igem.org/Team:MIT/ResultsFoundational#SDbio">Foundational page</a>.
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<h2>BBa_K779607: Tre-Tight-mKate inducible fluorescence with DOX</h2>
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<h2>BBa_K779400: U6-TetO-FF1 inducible RNA expression</h2>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=MIT" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="27551"  /><input type="hidden" name="pgroup" value="iGEM2012"  /><input type="hidden" name="group" value="MIT"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K779400'>BBa_K779400</A></TD><TD>Regulatory</TD><TD>U6-TetO-FF1 MammoBlock</TD><TD width='100px'>Divya Israni</TD><TD ALIGN='right'>326</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=MIT" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="27598"  /><input type="hidden" name="pgroup" value="iGEM2012"  /><input type="hidden" name="group" value="MIT"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K779501'>BBa_K779501</A></TD><TD>Other</TD><TD>Long reporter top strand MammoBlock</TD><TD width='100px'>Kristjan Eerik Kaseniit</TD><TD ALIGN='right'>20</TD><TD class  ='blank_col'></TD></FORM>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=MIT" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="27578"  /><input type="hidden" name="pgroup" value="iGEM2012"  /><input type="hidden" name="group" value="MIT"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K779607'>BBa_K779607</A></TD><TD>Generator</TD><TD>Tre-Tight-mKate MammoBlock Device</TD><TD width='100px'>Katie Bodner</TD><TD ALIGN='right'>939</TD><TD class  ='blank_col'></TD></FORM></TABLE></FORM><BR><BR>
<div class= "section" id="b2bio">
<div class= "section" id="b2bio">

Revision as of 03:23, 4 October 2012

DEPRECATED. DO NOT USE OR EDIT. If a page uses this template, relink with MIT-results2.

Our Favorite BioBricks

Favorite MIT 2012 iGEM Team PartsEdit
-?-NameTypeDescriptionDesignerLength
 WBBa_K779400RegulatoryU6-TetO-FF1 MammoBlockDivya Israni326
 WBBa_K779501OtherLong reporter top strand MammoBlockKristjan Eerik Kaseniit20
 WBBa_K779607GeneratorTre-Tight-mKate MammoBlock DeviceKatie Bodner939


All BioBricks

NameTypeDescriptionDesignerLength
BBa_K779100DNAFuel 1 - mRNA domain 1 DNA sensorDivya Israni18
BBa_K779101DNAGate 1 - mRNA domain 1 DNA sensorDivya Israni25
BBa_K779102DNAOutput 1 - mRNA domain 1 DNA sensorDivya Israni33
BBa_K779103DNAInput Mimic 1 - mRNA domain 1 DNA sensorDivya Israni20
BBa_K779104DNAFuel 2 - mRNA domain 2 DNA sensorDivya Israni18
BBa_K779105DNAGate 2 - mRNA domain 2 DNA sensorDivya Israni25
BBa_K779106DNAOutput 2 - mRNA domain 2 DNA sensorDivya Israni33
BBa_K779107DNAInput Mimic 2 - mRNA domain 2 DNA sensorDivya Israni20
BBa_K779108DNAFuel 3 - mRNA domain 3 DNA sensorDivya Israni18
BBa_K779109DNAGate 3 - mRNA domain 3 DNA sensorDivya Israni25
BBa_K779110DNAOutput 3 - mRNA domain 3 DNA sensorDivya Israni33
BBa_K779111DNAInput Mimic 3 - mRNA domain 3 DNA sensorDivya Israni20
BBa_K779112DNAFuel 4 - mRNA domain 4 DNA sensorDivya Israni18
BBa_K779113DNAGate 4 - mRNA domain 4 DNA sensorDivya Israni33
BBa_K779114DNAOutput 4 - mRNA domain 4 DNA sensorDivya Israni25
BBa_K779115DNAInput Mimic 4 - mRNA domain 4 DNA sensorDivya Israni20
BBa_K779116RNAShort RNA reporter bottom strand (with ROX fluorophore) Divya Israni20
BBa_K779117RNARNA Reporter top strand with quencher (RQ)Divya Israni15
BBa_K779118RNARNA Input S6Divya Israni20
BBa_K779119RNARNA Control input (scrambled)Divya Israni20
BBa_K779120RNARNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488)Divya Israni15
BBa_K779121DNAShort DNA reporter top strand (with RQ quencher)Divya Israni15
BBa_K779122DNAShort DNA reporter bottom strand (with ROX fluorophore)Divya Israni20
BBa_K779123DNAa - In vitro NOT gateDivya Israni43
BBa_K779124DNAb1 - In vitro NOT gateDivya Israni28
BBa_K779125DNAb2 - In vitro NOT gateDivya Israni20
BBa_K779126DNAc1 - In vitro NOT gateDivya Israni43
BBa_K779127DNAc2 - In vitro NOT gateDivya Israni29
BBa_K779128DNAc3 - In vitro NOT gateDivya Israni47
BBa_K779129DNAd1 - In vitro NOT gateDivya Israni29
BBa_K779130DNAd2 - In vitro NOT gateDivya Israni38
BBa_K779131DNAe - In vitro NOT gateDivya Israni34
BBa_K779200RegulatoryHef1AGiulio Alighieri1174
BBa_K779201RegulatoryCMVGiulio Alighieri70
BBa_K779202RegulatoryTRE-TightGiulio Alighieri196
BBa_K779300ReportereBFP2Giulio Alighieri726
BBa_K779301ReportereYFPGiulio Alighieri726
BBa_K779302ReportereYFP-4xFF1Giulio Alighieri859
BBa_K779303ReportereYFP-4xFF4Giulio Alighieri835
BBa_K779304ReportermKateGiulio Alighieri721
BBa_K779305CodingrtTA3Giulio Alighieri714
BBa_K779306ReporterTagBFPKatie Bodner699
BBa_K779307ReporterTagBFP-FF5Katie Bodner801
BBa_K779308CodingTetRKatie Bodner630
BBa_K779309ReportereBFP2-1xKatie Bodner754
BBa_K779310ReportereBFP2-2xKatie Bodner778
BBa_K779311ReporterHH-mKate - Hammerhead attached to front of mKateKatie Bodner802
BBa_K779312ReportermKate-HH - Hammerhead attached behind mKateKatie Bodner804
BBa_K779313ReporterHHM-mKate - Repressible hammerhead attached to front of mKateFelix Sun838
BBa_K779314ReportermKate-HHm - Repressible hammerhead behind mKateFelix Sun844
BBa_K779315Coding5' Hammerhead with loss-of-function mutationFelix Sun190
BBa_K779316Coding3' Hammerhead with loss-of-function mutationFelix Sun186
BBa_K779317Coding5' HammerheadFelix Sun190
BBa_K779318Coding3' HammerheadFelix Sun186
BBa_K779400RegulatoryU6-TetO-FF1Divya Israni326
BBa_K779401RegulatoryU6-TetO-S6Divya Israni277
BBa_K779402RegulatoryU6-TetO-S1Divya Israni277
BBa_K779403RegulatoryU6-TetO-FF4Divya Israni596
BBa_K779404RegulatoryU6-TetO-DecoyFF4-3pDivya Israni287
BBa_K779405RegulatoryU6-TetO-DecoyFF4-3p-4ntinDivya Israni291
BBa_K779406RegulatoryU6-TetO-TuDFF4-3pDivya Israni390
BBa_K779407RegulatoryU6-TetO-TuDFF4-3p-4ntinDivya Israni398
BBa_K779408RegulatoryU6-TetO-SaDivya Israni285
BBa_K779409RegulatoryU6-TetO-ScDivya Israni285
BBa_K779500OtherLong reporter top strand with bulgeKristjan Eerik Kaseniit24
BBa_K779501OtherLong reporter top strandKristjan Eerik Kaseniit20
BBa_K779502OtherLong reporter bottom strandKristjan Eerik Kaseniit28
BBa_K779503OtherLong input strandKristjan Eerik Kaseniit28
BBa_K779504OtherLong scrambled input strandKristjan Eerik Kaseniit28
BBa_K779600GeneratorHef1a-eYFPGiulio Alighieri1922
BBa_K779601GeneratorHef1a-mKateGiulio Alighieri1917
BBa_K779602GeneratorHef1a-TagBFP-FF5Giulio Alighieri1997
BBa_K779603GeneratorHef1a-eYFP-4xFF1Giulio Alighieri1922
BBa_K779604GeneratorHef1a-rTTA3Giulio Alighieri1926
BBa_K779605GeneratorHef1A-TetRKatie Bodner1842
BBa_K779606GeneratorTre-T-eYFP Katie Bodner944
BBa_K779607GeneratorTre-Tight-mKateKatie Bodner939