Team:MIT/ResultsActuation

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Revision as of 01:28, 2 October 2012

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Decoys and Tough Decoys (TuDs)

We wanted something that would provide a tight double repression system with a very distinct change between on and off. The TuDs and Decoys design were originally inspired by the “Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells,” paper. We copied their designs and wanted to reproduce the results in our lab. To do so, we ordered TuDs and decoys both with and without bulges. The bulges are designed to disrupt RISC complex activity; something which degrades short RNA like our decoys in the cell.
Sources: http://nar.oxfordjournals.org/content/early/2009/02/17/nar.gkp040.abstract
http://www.ncbi.nlm.nih.gov/pubmed/9695408