Team:MIT/Motivation

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Qian and Winfree (<i>Science</i> 2011) utilized DNA computation to create AND and OR logic gates <i>in vitro</i>. They constructed a sophisticated binary square root circuit using these gates:
Qian and Winfree (<i>Science</i> 2011) utilized DNA computation to create AND and OR logic gates <i>in vitro</i>. They constructed a sophisticated binary square root circuit using these gates:
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Revision as of 02:11, 27 October 2012

iGEM 2012

Background and Motivation

In the near future, biological circuits will be much more modular and sophisticated than they are now, with a ten-fold smaller nucleotide footprint.

The Enabling Technology: Toehold-Mediated Strand Displacement

A gate strand and output strand exist as a complex that is partially bound through complementary Watson-Crick base-pairing within the S2 binding domain. The gate strand also contains an open, unbound domain called a toehold region, T*. An input strand with a free complementary toehold region, T, can bind to the toehold region on the gate strand, and subsequently displace the output strand to yield an input-gate complex. This is called a toehold-mediated strand displacement reaction. The output strand is used as an input for a downstream gate-output complex.

Background

Qian and Winfree (Science 2011) utilized DNA computation to create AND and OR logic gates in vitro. They constructed a sophisticated binary square root circuit using these gates:

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