Team:MIT/MaterialsAndMethods

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iGEM 2012

Bacterial

  • Placeholder1
  • Placeholder2

In Vitro

  • Gate Anneals
  • Plate Reader Studies

Mammalian

  • DNA Transfection
  • RNA Transfection

Gate Anneals

Room Temperature Annealing
  1.  Mix gate and output in a 1.2:1 ratio in a tube.
  2.  Cover the tube in foil, if you didn't use an opaque/black tube.
  3.  Leave the tube on a bench top overnight.

Plate Reader Studies

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT