Team:MIT

From 2012.igem.org

(Difference between revisions)
(Prototype team page)
m
 
(102 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{MIT-style}}{{MIT-header2}}<html>
 +
<head>
 +
<script type="text/javascript" src="http://konami-js.googlecode.com/svn/trunk/konami.js"></script>
 +
<script>
-
<html>
+
konami = new Konami();
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
document.title = "MIT iGEM - Home";
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
konami.load("http://gobarbra.com/hit/new-93fa9e27c975c039c9eadefa578aa2be");
-
This is a template page. READ THESE INSTRUCTIONS.
+
</script>
 +
</head>
 +
<body>
 +
<div id="col_center">
 +
    <div id="block-content">
 +
    </div><!--end block-content-->
 +
 
 +
 
 +
<div style="line-height: 220px">
 +
<a href="https://2012.igem.org/Team:MIT/Motivation#s2">
 +
  <img src="https://static.igem.org/mediawiki/2012/4/44/Mithomepage1.png" alt="Why make logic circuits with strand displacement?" style="border:1px solid black" width="250"/>
 +
</a>
 +
 
 +
<a href="https://2012.igem.org/Team:MIT/Motivation">
 +
  <img src="https://static.igem.org/mediawiki/2012/5/52/Mithomepage3.png" alt="How does strand displacement work?" style="border:1px solid black" width="250"/>
 +
</a>
 +
 
 +
<a href="https://2012.igem.org/Team:MIT/TheKeyReaction#SDbio">
 +
  <img src="https://static.igem.org/mediawiki/2012/d/dd/Mithomepage5.png" alt="Strand displacement reactions work in vivo!" style="border:1px solid black" width="250"/>
 +
</a>
 +
<br />
 +
 
 +
<a href="https://2012.igem.org/Team:MIT/Sensing#sensing1bio">
 +
  <img src="https://static.igem.org/mediawiki/2012/8/81/Mithomepage4.png" alt="Sensing mRNA Levels using Strand Displacement" style="border:1px solid black" width="250"/>
 +
</a>
 +
 
 +
<a href="https://2012.igem.org/Team:MIT/NOTGate#NOTgate_designbio">
 +
  <img src="https://static.igem.org/mediawiki/2012/b/b7/Mithomepage6.png" alt="Strand Displacement NOT Gate Design" style="border:1px solid black" width="250"/>
 +
</a>
 +
 
 +
<a href="https://2012.igem.org/Team:MIT/CircuitProduction#shortRNAbio">
 +
  <img src="https://static.igem.org/mediawiki/2012/b/b0/Mithomepage2.png" alt="Making short RNAs in vivo to use in circuits" style="border:1px solid black" width="250"/><br/>
 +
</a>
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
 
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
<h3>Project Description</h3>
 +
<div id = "center">
 +
<p>
 +
<p>The limited availability of promoters, genes, and repressors, along with the difficulty in assembling and delivering large DNA plasmids bottleneck advances in sophistication of genetic circuits in mammalian systems. In contrast, sophistication of <i>in vitro</i> synthetic DNA circuits has grown exponentially through the mechanism of <a href="https://2012.igem.org/Team:MIT/Motivation">toehold-mediated strand displacement</a>. These circuits demonstrate digital logic with reliable, modular, and scalable behaviors and maintain a small base-pair footprint. </p> <p>
 +
The raw processing power of these strand displacement circuits has been trapped in the test tube, sequestered from the traditional protein-based sensing, processing, and actuation method of synthetic biology. With the adaptation of strand displacement-based information processing, the application space of synthetic biology circuits will become larger and more accessible. </p>
 +
 
 +
<p>
 +
Our project leverages strand displacement to create a processing technology that supports multi-input <b>sensing</b>, sophisticated <b>information processing</b>, and precisely-regulated <b>actuation</b> in mammalian cells. We designed and tested a novel <a href="https://2012.igem.org/Team:MIT/NOTGate">fully-functioning DNA NOT gate</a>, which enables complete logic operation. In addition, we used RNA strand displacement to <a href="https://2012.igem.org/Team:MIT/Sensing">sense cellular mRNA</a>. We also demonstrated our ability to <a href="https://2012.igem.org/Team:MIT/CircuitProduction#shortRNAbio">produce short RNAs</a> <i>in vivo</i>.
 +
 
 +
</p>
 +
 
 +
<p>
 +
Most importantly, we demonstrated that toehold-mediated strand displacement in RNA can occur in mammalian cells. This, combined with our feasibility studies outlined in the above paragraph, shows that <a href="https://2012.igem.org/Team:MIT/TheKeyReaction#iteration_2_invivo"><b>strand displacement is a viable information-processing technology</b></a>. We envision <i>in vivo</i> RNA strand displacement as a new foundation for scaling up complexity in engineered biological systems, with applications in biosynthesis, biomedical diagnostics and therapeutics.
 +
 
 +
</p>
 +
 
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
 
-
</div>
 
-
</div>
 
-
</html>
 
-
<!-- *** End of the alert box *** -->
 
 +
<br /><br />
-
{|align="justify"
+
<h3>Sponsors</h3>
-
|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
+
<ul id="leftsponsors">
-
|[[Image:MIT_logo.png|200px|right|frame]]
+
  <li><a href="http://www.eecs.mit.edu/"><img src='https://static.igem.org/mediawiki/2011/2/22/Mit-eecs.jpg' /></a></li>
-
|-
+
  <li><a href="http://web.mit.edu/be/"><img src='https://static.igem.org/mediawiki/2011/a/a7/Mit-be.jpg' /></a></li>
-
|
+
  <li><a href="http://web.mit.edu/cheme/"><img src='https://static.igem.org/mediawiki/2012/0/01/Cheme.png' style="width:175px"></a></li>
-
''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
  <li><a href="https://2012.igem.org/Main_Page"><img src='https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png' style="width:175px;"></a></li>
-
|[[Image:MIT_team.png|right|frame|Your team picture]]
+
  <li><a href="http://www.alnylam.com"><img src='https://static.igem.org/mediawiki/2012/1/16/ALYNYAM.jpg' style = "width:175px;"'></a></li>
-
|-
+
</ul>
-
|
+
-
|align="center"|[[Team:MIT | Team MIT]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
+
<ul id="centersponsors">
 +
  <li><a href="http://www.geneious.com"><img src='https://static.igem.org/mediawiki/2011/6/65/Mit-geneious.jpg' /></a></li>
 +
  <li><a href="http://www.genewiz.com"><img src='https://static.igem.org/mediawiki/2011/3/33/Mit-genewiz.jpg' /></a></li>
 +
  <li><a href="http://www.neb.com"><img src='https://static.igem.org/mediawiki/2011/4/4d/Mit-neb.jpg' /></a></li>
 +
  <li><a href="http://www.addgene.org/"><img src='https://static.igem.org/mediawiki/2012/5/58/Addgene.png' /></a></li>
 +
  <li><a href="http://www.genscript.com/"><img src='https://static.igem.org/mediawiki/2012/8/80/Genscript_logo2.jpg' /></a>
 +
</li>
 +
  <li><a href="http://www.pfizer.com"><img src='http://upload.wikimedia.org/wikipedia/en/7/77/Pfizerlogo.gif' width=175></a></li>
 +
</ul>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<ul id="rightsponsors">
-
!align="center"|[[Team:MIT|Home]]
+
  <li><a href="http://ebics.net"><img src='https://static.igem.org/mediawiki/igem.org/0/0d/EBICS_logo.JPG' style="width:175px"></a></li>
-
!align="center"|[[Team:MIT/Team|Team]]
+
  <li><a href="http://ehs.mit.edu/site/"><img src='https://static.igem.org/mediawiki/2012/5/52/Ehs_logo.jpg'></a></li>
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=MIT Official Team Profile]
+
  <li><a href="http://www.monsanto.com/Pages/default.aspx"><img src='https://static.igem.org/mediawiki/2012/1/18/Monsanto.png'></a></li>
-
!align="center"|[[Team:MIT/Project|Project]]
+
  <li><a href="http://www.flagshipventures.com/"><img src='https://static.igem.org/mediawiki/2012/3/3c/VentureLabsLogo.jpeg' style="width:175px"></a></li>
-
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
+
  <li><a href="http://www.thirdrockventures.com"><img src='https://static.igem.org/mediawiki/2012/c/ca/TRV.jpg' style="width:175px"></a></li>
-
!align="center"|[[Team:MIT/Modeling|Modeling]]
+
  <li><a href="http://www.idtdna.com/site"><img src='https://static.igem.org/mediawiki/2012/4/41/Idt2.png'></a></li>
-
!align="center"|[[Team:MIT/Notebook|Notebook]]
+
  <li><a href="http://www.cmea.com/team/team-karl-handelsman.php"><img src='https://static.igem.org/mediawiki/2012/f/f0/Karl_Handelsman_logo.png' width=175> </a></li>
-
!align="center"|[[Team:MIT/Safety|Safety]]
+
 
-
!align="center"|[[Team:MIT/Attributions|Attributions]]
+
 
-
|}
+
</ul>
 +
 
 +
<br />
 +
 
 +
   
 +
 
 +
</div> <!--End of col_center-->
 +
 +
</body>
 +
</html>

Latest revision as of 19:41, 25 June 2013

iGEM 2012
Why make logic circuits with strand displacement? How does strand displacement work? Strand displacement reactions work in vivo!
Sensing mRNA Levels using Strand Displacement Strand Displacement NOT Gate Design Making short RNAs in vivo to use in circuits

Project Description

The limited availability of promoters, genes, and repressors, along with the difficulty in assembling and delivering large DNA plasmids bottleneck advances in sophistication of genetic circuits in mammalian systems. In contrast, sophistication of in vitro synthetic DNA circuits has grown exponentially through the mechanism of toehold-mediated strand displacement. These circuits demonstrate digital logic with reliable, modular, and scalable behaviors and maintain a small base-pair footprint.

The raw processing power of these strand displacement circuits has been trapped in the test tube, sequestered from the traditional protein-based sensing, processing, and actuation method of synthetic biology. With the adaptation of strand displacement-based information processing, the application space of synthetic biology circuits will become larger and more accessible.

Our project leverages strand displacement to create a processing technology that supports multi-input sensing, sophisticated information processing, and precisely-regulated actuation in mammalian cells. We designed and tested a novel fully-functioning DNA NOT gate, which enables complete logic operation. In addition, we used RNA strand displacement to sense cellular mRNA. We also demonstrated our ability to produce short RNAs in vivo.

Most importantly, we demonstrated that toehold-mediated strand displacement in RNA can occur in mammalian cells. This, combined with our feasibility studies outlined in the above paragraph, shows that strand displacement is a viable information-processing technology. We envision in vivo RNA strand displacement as a new foundation for scaling up complexity in engineered biological systems, with applications in biosynthesis, biomedical diagnostics and therapeutics.



Sponsors