http://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&feed=atom&action=historyTeam:Lyon-INSA/safety - Revision history2024-03-29T01:32:19ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=301016&oldid=prevXavier69 at 12:03, 8 June 20132013-06-08T12:03:43Z<p></p>
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</table>Xavier69http://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=301008&oldid=prevXavier69 at 11:56, 8 June 20132013-06-08T11:56:02Z<p></p>
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</table>Xavier69http://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=292850&oldid=prevAlmiwe at 00:10, 27 October 20122012-10-27T00:10:08Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “Biofilm Killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 µm)(<a href="https://2012.igem.org/Team:Lyon-INSA/BiofilmK"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “Biofilm Killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 µm)(<a href="https://2012.igem.org/Team:Lyon-INSA/BiofilmK<ins class="diffchange diffchange-inline">#solution</ins>"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td></tr>
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</table>Almiwehttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=292708&oldid=prevSuxiaohui at 00:03, 27 October 20122012-10-27T00:03:46Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=292708&oldid=292039">Show changes</a>Suxiaohuihttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=292039&oldid=prevAlmiwe at 23:32, 26 October 20122012-10-26T23:32:19Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron)(<a href="https://2012.igem.org/Team:Lyon-INSA/BiofilmK"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron)(<a href="https://2012.igem.org/Team:Lyon-INSA/BiofilmK<ins class="diffchange diffchange-inline">#solution</ins>"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td></tr>
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</table>Almiwehttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=289915&oldid=prevAudreyM at 21:21, 26 October 20122012-10-26T21:21:43Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron)(<a href="<del class="diffchange diffchange-inline">http://</del>https://2012.igem.org/Team:Lyon-INSA/BiofilmK"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron)(<a href="https://2012.igem.org/Team:Lyon-INSA/BiofilmK"> See process in "Our solution"</a>)<br/><br/>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td></tr>
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</table>AudreyMhttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=289868&oldid=prevAudreyM at 21:18, 26 October 20122012-10-26T21:18:40Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron). <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (double filter 0.45/0.2 micron)<ins class="diffchange diffchange-inline">(<a href="http://https://2012.igem.org/Team:Lyon-INSA/BiofilmK"> See process in "Our solution"</a>)<br/><br/></ins>. <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td></tr>
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</table>AudreyMhttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=289560&oldid=prevCdorelflamant at 20:54, 26 October 20122012-10-26T20:54:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (<del class="diffchange diffchange-inline">mettre un lien vers industrialization, </del>filter 0.45 0.2 micron). <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> <del class="diffchange diffchange-inline">(lien)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>B. subtilis</i> is the chassis chosen for “biofilm killer”. <i>B. subtilis</i> is widely used in the field as a biocontrol agent against plant pathogens and food industry processes. It has been granted Qualified Presumption of Safety status by the European Food Safety Authority (EFSA). However, if modified by biobricks, <i>B. subtilis</i> has to be physically contained (<ins class="diffchange diffchange-inline">double </ins>filter 0.45<ins class="diffchange diffchange-inline">/</ins>0.2 micron). <strong>Enhanced safety can be achieved by inserting functions in the chassis that prevent horizontal tranfert of DNA from our bacterium to another one. This aspect is developed in the chapter "New ideas for safety in iGEM"</strong> </div></td></tr>
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</table>Cdorelflamanthttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=289355&oldid=prevCdorelflamant at 20:40, 26 October 20122012-10-26T20:40:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Protection</strong><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Protection</strong><br/><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Some of the reagents used are irritant, toxic and can be potential carcinogens (<del class="diffchange diffchange-inline">agarose, </del>polyacrylamid<del class="diffchange diffchange-inline">, methanol</del>, Ethidium bromide…). To minimize the impact of their use, they are manipulated following the supplier’s instructions, wearing appropriate personal safety equipment, i.e. gloves, safety glasses, labcoats and under extractor hood when necessary. All samples, tubes, vials are clearly identified/labeled to avoid inappropriate mix between two non compatible solvents.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Some of the reagents used are irritant, toxic and can be potential carcinogens (polyacrylamid, Ethidium bromide…). To minimize the impact of their use, they are manipulated following the supplier’s instructions, wearing appropriate personal safety equipment, i.e. gloves, safety glasses, labcoats and under extractor hood when necessary. All samples, tubes, vials are clearly identified/labeled to avoid inappropriate mix between two non compatible solvents.</div></td></tr>
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</table>Cdorelflamanthttp://2012.igem.org/wiki/index.php?title=Team:Lyon-INSA/safety&diff=289318&oldid=prevCdorelflamant at 20:37, 26 October 20122012-10-26T20:37:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Safety</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Safety</h1></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We present here our reflexion about safety issues of the “Biofilm Killer” project based on a modified <i>Bacillus subtilis</i> strain able to swarm into biofilms, to produce a biocide agent and a dispersive agent. To obtain genetic constructions, we worked with an <i>Escherichia coli</i> strain<del class="diffchange diffchange-inline">, </del><i>Staphylococcus epidermidis</i>, <i>S. aureus</i> and adherent <i>E. coli</i> strains were used as biofilm models. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We present here our reflexion about safety issues of the “Biofilm Killer” project based on a modified <i>Bacillus subtilis</i> strain able to swarm into biofilms, to produce a biocide agent and a dispersive agent. To obtain genetic constructions, we worked with an <i>Escherichia coli</i> strain<ins class="diffchange diffchange-inline">. </ins><i>Staphylococcus epidermidis</i>, <i>S. aureus</i> and adherent <i>E. coli</i> strains were used as biofilm models. </div></td></tr>
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</table>Cdorelflamant