Experience n11st June 2012Une experience trop cooltitre 2nd June 2012blablaExperience n21st July 2012Une experience pas coolFor all purposes Monday, July 2nd 2012Liquid culture (5 mL LB media) of NM 522 cells and overnight incubation under agitation at 37°C for later transformations.For all purposes Tuesday, July 3rd 2012Dilution of 100 µL saturated culture in 5 mL LB media.
Incubation time : 2 hours (until O.D =0,3).
Transformation of the NM 522 strain (this experiment was made 3 times)
For the positive control the pSB1C3 plasmid was used ;
For the transformation, the pBBA_I742123 was used (well A2, 5th iGEM kit plate)
The transformed bacteria were selected on chloramphenicol plates.
"
