Team:Lyon-INSA/notebook

From 2012.igem.org

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<jour nb="31">
<jour nb="31">
                         <date>Friday, August 31st 2012</date>
                         <date>Friday, August 31st 2012</date>
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                        <titre>For all purposes</titre>
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<description>
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We have finally received the enzymes, so we tested the minipreps containing the modified shuttle vectors (containing the iGEM linker). The results are promising : 4 plasmids are digested by SpeI, so the ligation was successful. The four plasmids are further tested for the other 3 iGem sites. To make sure that the site SpeI is not the initial site that we eliminated by filling-in and that the plasmid used for transformation and ligation was not contaminated with the original plasmid, a double digested was done (EcoRI and SpeI). There was no fragment at 1000 pb, so the ligation was successful.
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</description>
                         <titre>Kill</titre>
                         <titre>Kill</titre>
<description>
<description>
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</description>
</description>
                       <titre>Surfactant</titre>
                       <titre>Surfactant</titre>
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<description>
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miniprep of the saturated cultures with transformed bacteria containing the ligation of the sfp and abrB genes. The electrophoresis confirmed that this time the bacteria contained the rigth plasmids.
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Miniprep of the saturated cultures with transformed bacteria containing the ligation of the sfp and abrB genes. The electrophoresis confirmed that this time the bacteria contained the right plasmids.
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</description>
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Stick :
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                      </jour>
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</month>
</month>

Revision as of 15:19, 11 September 2012


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