Team:Lyon-INSA/notebook

From 2012.igem.org

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</description>
</description>
                       </jour>
                       </jour>
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___________________________________________________________________________________
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Friday, 17th August :
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<jour nb="17">
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For all :
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                        <date>Friday, August 17th 2012</date>
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- The plasmids (pBK19 and pBK20) from 2 clones were re-extracted using columns. This time the digestion was successful. The tests showed that there was no SpeI site. However, the pHT315 plasmid also had no XbaI site.
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                        <titre>For all purposes</titre>
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Stick :
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<description>
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- Plasmid extractions from the 14 clones transformed by PBK7+XylR (with the second ligation). Like for the first transformation, the electrophoresis of the digested plasmids showed that the clones have not the right plasmid : they have just the vector without XylR.  
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The plasmids (pBK19 and pBK20) from 2 clones were re-extracted using columns. This time the digestion was successful. The tests showed that there was no SpeI site. However, the pHT315 plasmid also had no XbaI site.
 +
</description>
 +
                      <titre>Stick</titre>
 +
<description>
 +
Plasmid extractions from the 14 clones transformed by [pBK7 + XylR] (with the second ligation). Like for the first transformation, the electrophoresis of the digested plasmids showed that the clones do not have the right plasmid : they have just the vector without XylR.
 +
</description>
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                      </jour>

Revision as of 16:55, 10 September 2012


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