Team:Lyon-INSA/notebook

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       <li>Cloning of the constitutive promoter in front of the dispersin part (the gene coding for Dispersin) and RBS/GFP (of <i>E. coli</i>) using a 3A ligation followed by transformation of the ligation in the NM522 strain;</li>
       <li>Cloning of the constitutive promoter in front of the dispersin part (the gene coding for Dispersin) and RBS/GFP (of <i>E. coli</i>) using a 3A ligation followed by transformation of the ligation in the NM522 strain;</li>
       <li>Extraction of pUC57-lysostaphin. Gel electrophoresis showed a shaded DNA → RNase addition in the extraction kit buffer was forgotten.</li>
       <li>Extraction of pUC57-lysostaphin. Gel electrophoresis showed a shaded DNA → RNase addition in the extraction kit buffer was forgotten.</li>
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</ul>
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</description>
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<titre>Physiological tests : Testing the potential of S. epidermidis to form biofilms</titre>
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<description>
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<ul>
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<li>Liquid culture of <i>S. epidermidis</i> in 5mL of TSB (conditions described in protocol “Tests on Staphylococcus aureus biofilms in 24 well plate” ).</li>
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<li>A bacterial suspension of <i>S. epidermidis</i> with OD<sub>600</sub> close to 0.132 is prepared from a Petri dish containing <i>S. epidermidis</i>. A microtiter plate is inoculated with 2 mL of the suspension  diluted 1:100 with TSB supplemented with different concentrations of glucose in order to test the best conditions for biofilm formation. The plate is incubated during 24 hours at 37ºC.</li>
</ul>
</ul>
</description>
</description>
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</jour>
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<jour nb="18">
</jour>
</jour>

Revision as of 04:54, 26 September 2012


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