From 2012.igem.org
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Revision as of 14:29, 21 August 2012
Experience n1
1st June 2012
Une experience trop cool
titre
2nd June 2012
blabla
Experience n2
1st July 2012
Une experience pas cool
For all purposes
Monday, July 2nd 2012
Liquid culture (5 mL LB media) of NM 522 cells and overnight incubation under agitation at 37°C for later transformations.
For all purposes
Tuesday, July 3rd 2012
Dilution of 100 µL saturated culture in 5 mL LB media.
Incubation time : 2 hours (until O.D =0,3).
Transformation of the NM 522 strain (this experiment was made 3 times)
For the positive control the pSB1C3 plasmid was used ;
For the transformation, the pBBA_I742123 was used (well A2, 5th iGEM kit plate)
The transformed bacteria were selected on chloramphenicol plates.
June
July
August
September
October
"
