Team:Lyon-INSA/notebook

From 2012.igem.org

(Difference between revisions)
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<titre>Surfactant</titre>
<titre>Surfactant</titre>
<description>
<description>
 +
Screening of 15 clones per transformation (antibiotic resistance test)
</description>
</description>
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<titre>Surfactant</titre>
<titre>Surfactant</titre>
<description>
<description>
 +
Liquid cultures are inoculated with colonies having the expected antibiotic phenotype.
 +
</br>
To test the surfactant properties of the surfactin produced by the <i>B. subtilis</i> BK52 strain, a biofilm test was made in a 24 well plate. Liquid cultures are seeded with BK52 and BK49.
To test the surfactant properties of the surfactin produced by the <i>B. subtilis</i> BK52 strain, a biofilm test was made in a 24 well plate. Liquid cultures are seeded with BK52 and BK49.
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<titre>Surfactant</titre>
<titre>Surfactant</titre>
<description>
<description>
 +
Miniprep of the screened clones. Unfortunately, the gel electrophoresis was disappointing and the extracted plasmids did not contain the ligated genes.
 +
</br>
A 24-well plate is incubated with filtered supernatant of the saturated cultures with BK52 and BK49 at room temperature for 24 hours.
A 24-well plate is incubated with filtered supernatant of the saturated cultures with BK52 and BK49 at room temperature for 24 hours.
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<titre>Surfactant</titre>
<titre>Surfactant</titre>
<description>
<description>
-
 
+
Liquid cultures were inoculated with the strains BK49 and BK52 and then incubated at 37C for 48 hours.
</description>
</description>
<titre>Stick</titre>
<titre>Stick</titre>

Revision as of 20:32, 26 October 2012

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