Team:Lyon-INSA/notebook

From 2012.igem.org

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       <ul>
       <ul>
             <li>[Lysostaphin + Dispersin] in pSB1C3;</li>
             <li>[Lysostaphin + Dispersin] in pSB1C3;</li>
-
             <li>[Lysostaphin + Dispersin] in the shuttle vector (vector for <i>E. coli</i> and <i>B. subtilis,/i>).</li>
+
             <li>[Lysostaphin + Dispersin] in the shuttle vector (vector for <i>E. coli</i> and <i>B. subtilis,</i>).</li>
       </ul>
       </ul>
</ul>
</ul>
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<ul>
<ul>
<li>Extraction of the good clone from the replica-printing. Then a electrophoresis gel is run : the blunt end ligation was successful.</li>
<li>Extraction of the good clone from the replica-printing. Then a electrophoresis gel is run : the blunt end ligation was successful.</li>
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<li>The plasmid is digested by <i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I and <i>Pst</i>I to find out if the problem comes from a bad site sequence. Result : the <i>Eco</i>RI restriction site is not good, therefore is not recognized by <i>Eco</i<RI enzyme.</li>
+
<li>The plasmid is digested by <i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I and <i>Pst</i>I to find out if the problem comes from a bad site sequence. Result : the <i>Eco</i>RI restriction site is not good, therefore is not recognized by <i>Eco</i>RI enzyme.</li>
</ul>
</ul>
</description>
</description>

Revision as of 16:25, 26 September 2012


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