Team:LMU-Munich/Why Beadzillus

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==Why Beadzillus?==
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== The Filter Issue ==
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Filters are widely used in everyday life and within the lab. Filters, such as ''Brita'' filters for removing calcium and other contaminants from drinking water and plumbing systems are abundant. In the lab, filters (here usually refered to as columns) are used for DNA/protein purification and for protein characterization.</p>
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<font color="#000000"; size="2"><p align="justify"> Fig. 1: Filters for different applications. </p></font>
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The use of filters in every day life as well as in the lab is widespread. There are ''Brita'' filters to  filter chalk and other contaminants out of drinking water as well as for showers. In the lab filters are used for DNA purification or protein purification.
 
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PICTURE
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== Synthetic beads ==
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<p align="justify">Such filters usually consist of a cartridge that is filled with some type of matrix which determines the function of the filter. To maximize the surface in a minimal volume, microbeads are commonly used in all types of filters. These beads have a specific size and can for example be used to display protein on their surface. The coupling of proteins to the beads is based on affinity binding. An example are Ni-NTA resins (or beads) that can be used to bind proteins that harbour a His-tag. If a protein is bound this way to the beads, its features then determine the functional properties of such protein-loaded beads.
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All of the filters are filled with some matices which determine the function of the filter. To maximize the surface in a miminal volume, beads are commonly applied in any kind of filters. These microbeads have a certain size and can display protein on their surface for functional use of them. Then the feature of the protein characterize the characteristics of the filter. The coupling of proteins to the beads is based on affinity binding, for example nickle NTA-tags where the protein which carrys the histidin tag binds to the nickel ion.Several companies offer such microbeads with proteins coupled to their surface by using affinity tags.
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Such beads are usually quite expensive and coupling of proteins to the beads is laborious, as the protein has to  be expressed, bound to the beads and then washed. Additionally, the binding of the protein is non-covalent. </p>
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These beads are usually very expensive, the production is more labourious as the protein has to  be expressed, bound to the beads and then washed again, and the binding of the protein is non-covalent. For this problem, we want to offer a synthetic biology solution in our project '''BEAD'''zillus where we produced biological beads.
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== SynBio beads ==
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<p align="justify">To solve this problem, we have developed a synthetic biology solution in our project '''Bead'''zillus, in which biological beads display a protein of choice. We call these biological beads '''Sporo'''beads.
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But what are our biological beads made of? Using clever natural engineering millions of years ago, evolution developed endospores of the soil bacteria ''Bacillus subtilis''. Endospores, which are highly resistant to environmental stressors and can survive harsh conditions, are a dormant life stage of ''B. subtilis''. To get to know more about the life cycle and the production of endospores, have a look at the life cycle of ''B. subtilis'' on the next page.
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But what are these biological beads made of? Based on a clever thing, evolution developed millions of years ago endospores of the soil bacteria ''Bacillus subtilis''. They are a dormant life stage of this bacteria which is highly resistant and can survive harsh conditions. To get to know more about the life cycle and the production of endospores have a look at the life cycle of B. subtilis on the next page.
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/Bacillus_Introduction]]  
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/Data/differentiation_tour]] [[File:BACK.png|left|80px|link=Team:LMU-Munich/Abstract]]
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====Project Navigation====
 
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
 
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
 
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
 
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
 
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Latest revision as of 16:40, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Bacillus in urban culture.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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