Team:LMU-Munich/Spore Coat Proteins

From 2012.igem.org

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<p align="justify">Because of the low but distinct fluorescence of wildtype spores, we measured and compared the fluorescence intensity of 100 spores per construct (see [https://2012.igem.org/Team:LMU-Munich/Data/gfp_spore data]). The '''Sporo'''beads were investigated by fluorescence microscopy and analysed. We obtained significant differences between wildtype spores and all our '''Sporo'''beads [see https://2012.igem.org/Team:LMU-Munich/Data/gfp_spore Data]. The intensity bar charts should thereby show the fluorescence difference between wildtype (W168), B53- and B70-'''Sporo'''beads (B53 strain with native ''cotZ'' deletion). To demonstrate the distribution of the fusion proteins we created 3D graphs, which show the fluorescence intensity spread across the spore surface. For analysis we measured the fluorescence intensity of an area of 750px per spore by using ImageJ and evaluated it with the statistical software '''R'''. The following graph (Fig. 6) shows the results of microscopy and ImageJ analysis of the strongest construct integrated into wildtype W168 (B53) and the clean deletion mutant of ''cotZ'' (B 70).</p>   
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<p align="justify">Because of the low but distinct fluorescence of wildtype spores, we measured and compared the fluorescence intensity of 100 spores per construct (see [https://2012.igem.org/Team:LMU-Munich/Data/gfp_spore data]). The '''Sporo'''beads were investigated by fluorescence microscopy and analysed. We obtained significant differences between wildtype spores and all our '''Sporo'''beads (see [https://2012.igem.org/Team:LMU-Munich/Data/gfp_spore Data]). The intensity bar charts should thereby show the fluorescence difference between wildtype (W168), B53- and B70-'''Sporo'''beads (B53 strain with native ''cotZ'' deletion). To demonstrate the distribution of the fusion proteins we created 3D graphs, which show the fluorescence intensity spread across the spore surface. For analysis we measured the fluorescence intensity of an area of 750px per spore by using ImageJ and evaluated it with the statistical software '''R'''. The following graph (Fig. 6) shows the results of microscopy and ImageJ analysis of the strongest construct integrated into wildtype W168 (B53) and the clean deletion mutant of ''cotZ'' (B 70).</p>   

Revision as of 22:36, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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