Team:LMU-Munich/Spore Coat Proteins

From 2012.igem.org

(Difference between revisions)
Line 97: Line 97:
<p align="justify">The '''Sporo'''beads-Δ''cotZ'' were investigated by fluorescence microscopy and analysed like the other '''Sporo'''beads. The intensity bar charts should thereby show the fluorescence difference between wildtype (W168), B53- and B70-'''Sporo'''beads. To demonstrate the distribution of the fusion proteins we created 3D graphs, which show the fluorescence intensity spread across the spore surface. For analysis we measured the fluorescence intensity of a area of 750px per spore by using ImageJ and evaluated it with the statistical software '''R'''. The following graph shows the results of microscopy and ImageJ analysis.</p>   
<p align="justify">The '''Sporo'''beads-Δ''cotZ'' were investigated by fluorescence microscopy and analysed like the other '''Sporo'''beads. The intensity bar charts should thereby show the fluorescence difference between wildtype (W168), B53- and B70-'''Sporo'''beads. To demonstrate the distribution of the fusion proteins we created 3D graphs, which show the fluorescence intensity spread across the spore surface. For analysis we measured the fluorescence intensity of a area of 750px per spore by using ImageJ and evaluated it with the statistical software '''R'''. The following graph shows the results of microscopy and ImageJ analysis.</p>   
-
[[File:Example.jpg endbild]]
+
 
 +
{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{|
 +
|[[File:Fluorescence of Sporobeads.png|610px|center]]
 +
|}
 +
|-
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Result of fluorescence evaluation of the three strains: W168, B53 and B70.</font>
 +
|}
 +
|}
Line 111: Line 123:
</p>
</p>
-
<p align="justify">
 
-
B. subtilis spores are part of several dietary and health supplements and have a GRAS (Generally regarded as safe) status. Theoretically it might be possible to use the Sporobeads for dietary applications, but this includes several years of work and studying regulations. The [http://www.gesetze-im-internet.de/gcp-v/BJNR208100004.html GCP Verordnung] (“Good Clinical Practice ordinance”) has to be followed and the [http://www.pei.de/cln_101/DE/home/de-node.html?__nnn=true Paul-Ehrlich-Institute] would have to permit all the studies.
 
-
</p>
 
'''CPX:'''  
'''CPX:'''  
<p align="justify">
<p align="justify">
-
The [http://partsregistry.org/wiki/index.php/Part:BBa_I728500 CPX-peptide] generated by MIT (2007) can bind to Polystyrene which is a common plastic. The waste from Polystyrene is currently a big challenge in the field of environmental protection and remediation. In the ocean, large piece of Polystyrene litter are ground by sea currents into very small pieces, so called plastik plankton, that are consumed by fish, filter feeders, and other organisms living in the oceans. Such plastic uptake leads to poisoning, sterility and death. To remove Polystyrene from the oceans, huge filter boxes could be put into place to mechanically filter out large pieces; our CPX-Sporobeads could remove the microscopic plastic particles.  
+
The [http://partsregistry.org/wiki/index.php/Part:BBa_I728500 CPX-peptide] generated by MIT (2007) can bind to Polystyrene which is a common plastic. The waste from Polystyrene is currently a big challenge in the field of environmental protection and remediation. In the ocean, large pieces of Polystyrene litter are ground by sea currents into very small pieces, so called plastik plankton, that are consumed by fish, filter feeders, and other organisms living in the oceans. Such plastic uptake leads to poisoning, sterility and death. To remove Polystyrene from the oceans, huge filter boxes could be put into place to mechanically filter out large pieces; our CPX-Sporobeads could remove the microscopic plastic particles.  
</p>
</p>

Revision as of 15:19, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU eppis.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde