Team:LMU-Munich/Spore Coat Proteins

From 2012.igem.org

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=='''Sporo'''beads - What Protein Do ''You'' Want to Display?==  
=='''Sporo'''beads - What Protein Do ''You'' Want to Display?==  
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<p align="justify">'''Sporo'''beads are the first generation of ''Bacillus subtilis'' endospores displaying a protein of our choice on their outermost layer, the spore crust. In future '''Sporo'''beads could serve as a platform for protein display and thus be used for numerous versatile [https://2012.igem.org/Team:LMU-Munich/Application applications]. Our goal was to show that ''B. subtilis'' spores have the ability to do so. As a [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins/result proof of principle] we successfully fused GFP to the spore crust and obtained fluorescence with microscopy.</p> 
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===What are '''Sporo'''beads?===
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===Scientific Background===
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|<p align="justify">Introduction to ''B. subtilis'' spores  and their assignment for our project</p>
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|<p align="justify">Introduction to ''B. subtilis'' spores  and their use in our project</p>
|[[File:Imamura, 2011 &amp; McKenney, 2010.png|200px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/Background]]
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===Crust Promoter Evaluation===
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===Cloning Strategy===
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|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
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|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/crustpromoters]]
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===Crust Protein Gene Organization===
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|<p align="justify">Location of the two crust protein genes in the genome</p>
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|<p align="justify">Cloning strategy to create different variants of our ''' Sporo'''beads</p>
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|[[File:Operons.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/crustproteins]]
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|[[File:Final construct.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/crustproteins]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]]
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===Cloning Strategy===
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===GFP as a Proof of Principle===
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">Cloning strategy to create different variants of our ''' Sporo'''beads</p>
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|<p align="justify">Main results of the various constructs that were created to find the best one!</p>
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|[[File:Final construct.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]]
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|[[File:LMU Firstspore.jpg|200px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/result]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/result]]
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===Cloning Results===
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===Laccases as functional enzymes===
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">Main results of the various constructs that were created</p>
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|<p align="justify">Creation of functional Laccase-''' Sporo'''beads</p>
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|[[File:LMU Firstspore.jpg|150px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/result]]
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|[[File:Final construct.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/laccases]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/result]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/laccases]]
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====Project Navigation====
====Project Navigation====

Latest revision as of 13:44, 14 November 2012

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