Team:LMU-Munich/Spore Coat Proteins

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<p align="justify">Because of the low but distinct fluorescence of wildtype sores, we measured and compared the fluorescence intensity of 100 spores per mutant. As you can see in the following graph, a significant difference was obtained. However we worked with the PcotYZ-CotZ-GFP-Terminator spores for further experiments as these showed the brightest fluorescence. In these experiments we had three different aims.
<p align="justify">Because of the low but distinct fluorescence of wildtype sores, we measured and compared the fluorescence intensity of 100 spores per mutant. As you can see in the following graph, a significant difference was obtained. However we worked with the PcotYZ-CotZ-GFP-Terminator spores for further experiments as these showed the brightest fluorescence. In these experiments we had three different aims.
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The first one was to show that the fusion proteins are really located on the outermost layer. Therefore we investiagted the fluoerscence of our spores after treatment with proteinase K and FRAP (fluorescence recovery after photobleaching).</p>
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<br>The first one was to show that the fusion proteins are really located on the outermost layer. Therefore we investiagted the fluoerscence of our spores after treatment with proteinase K and FRAP (fluorescence recovery after photobleaching).
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<br>The second aim was to purify the '''Sporo'''beads from vegetative cells, which thereby should be deaden. We chose three different methods for this approach, the treatment with French Press, ultrasound (sonification) or lysozyme.    </p>
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Revision as of 11:47, 19 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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