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Team:LMU-Munich/Results
From 2012.igem.org
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| - | <font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 ( | + | <font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Mutants used were: <br /> |
| - | ''' | + | '''W168''': Wild-type 168 <br /> |
'''''spo0A''::tet''': A sporulation-deficient strain <br /> | '''''spo0A''::tet''': A sporulation-deficient strain <br /> | ||
'''B29''': ''cwlD''::kan, ''sleB''::mls <br /> | '''B29''': ''cwlD''::kan, ''sleB''::mls <br /> | ||
Revision as of 15:56, 26 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Here you will find the main results of our work.
B. subtilis vectors
| Name
BioBrick | E. coli res. | B. subt. res. | Insertion locus | Description | Cloning status | Works? |
|---|---|---|---|---|---|---|
| pSBBs1C (BBa_K823023) | Amp | Cm | amyE | empty |  | |
| pSBBs4S (BBa_K823022) | Amp | Spec | thrC | empty | | |
| pSBBs2E (BBa_K823027) | Amp | MLS | lacA | empty | in work | not tested |
| pSBBs1C-lacZ (BBa_K823021) | Amp | Cm | amyE | lacZ reporter | | |
| pSBBs3C-luxABCDE (BBa_K823025) | Amp | Cm | sacA | luxABCDE reporter | | |
| pSBBs4S-PXyl (BBa_K823024) | Amp | Spec | thrC | Xylose-promoter | | refuses transformation |
| pSBBs0K-Pspac (BBa_K823026) | Amp | Kan | replicative | IPTG-promoter | | unexpected |
| Sporovector (BBa_K823054) | Amp | Spec | thrC | to make Sporobeads | | not tested |
Overview of all evaluated promoters
This section gives an overview of all evaluated promoters which cover a large range of activity. For more details and informations of the experiments see the Data page of the promoters. Note that Pveg was not evaluated with luminescence measurements and this bar is just projected from the results of the beta-galactosidase assay.
|
Germination Gene Knockouts
|
Inverter
For detailed results visit our data page and for all background info our project page.
