Team:LMU-Munich/Results

From 2012.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 5: Line 5:
-
===Finished and evaluated 5 ''B. subtilis'' vectors in BioBrick standard!===
+
===Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.===
-
 
+
<br>
{| class="colored"
{| class="colored"
!Name  
!Name  
Line 84: Line 84:
-
===15 Promoters evaluated in ''B. subtilis'' and added to the registry===
+
===15 Promoters evaluated in ''B. subtilis'' and added to the registry!===
-
 
+
<br>
<p align="justify"> This section gives an overview on the strength of all evaluated promoters, which span a large range of activities. For more details and informations of the experiments see the [https://2012.igem.org/Team:LMU-Munich/Data Data] page of the promoters. Note that P<sub>''veg''</sub> was not evaluated with luminescence measurements and this bar is just projected from the results of the β-galactosidase assay.</p>
<p align="justify"> This section gives an overview on the strength of all evaluated promoters, which span a large range of activities. For more details and informations of the experiments see the [https://2012.igem.org/Team:LMU-Munich/Data Data] page of the promoters. Note that P<sub>''veg''</sub> was not evaluated with luminescence measurements and this bar is just projected from the results of the β-galactosidase assay.</p>
<br>
<br>
-
 
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
| style="width: 70%;background-color: #EBFCE4;" |
| style="width: 70%;background-color: #EBFCE4;" |
Line 103: Line 102:
 +
===Sporobeads - what protein do you want to display?===
 +
<br>
 +
{| style="color:black;" cellpadding="3" width="100%" cellspacing="0" border="0" align="center" style="text-align:left;"
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{|
 +
|[[File:Fluorescence of Sporobeads.png|610px|center]]
 +
|}
 +
|-
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2">Result of fluorescence evaluation of the three strains: W168, B53 and B70.</font>
 +
|}
 +
|}
-
===Erase<sup>*</sup> germination ability! Knockout strains===
 
 +
===Erase<sup>*</sup> germination ability! Knockout strains===
 +
<br>
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
| style="width: 70%;background-color: #EBFCE4;" |
| style="width: 70%;background-color: #EBFCE4;" |
Line 114: Line 128:
{| style="color:black;" cellpadding="3" width="95%" cellspacing="0" border="0" align="left" style="text-align:left;"
{| style="color:black;" cellpadding="3" width="95%" cellspacing="0" border="0" align="left" style="text-align:left;"
|style="width: 70%;background-color: #EBFCE4;" |
|style="width: 70%;background-color: #EBFCE4;" |
-
<font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Mutants used were: <br />
+
<font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. W168 showed near 100% germination rates. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Our results were consistent with this expectation. Mutants used were: <br />
-
'''W168''': Wild-type 168 <br />
+
'''W168''': wild-type 168 <br />
-
'''''spo0A''::tet''': A sporulation-deficient strain <br />
+
'''''spo0A''::tet''': a sporulation-deficient strain <br />
'''B29''': ''cwlD''::kan, ''sleB''::mls <br />
'''B29''': ''cwlD''::kan, ''sleB''::mls <br />
'''B30''': ''gerD''::cm, ''sleB''::mls <br />
'''B30''': ''gerD''::cm, ''sleB''::mls <br />
Line 125: Line 139:
'''B43''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec <br />
'''B43''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec <br />
'''B46''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm, ''sleB''::mls <br />
'''B46''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm, ''sleB''::mls <br />
-
'''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan</font>
+
'''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan<br></font>
|}
|}
|}
|}
|}
|}
-
<p align="justify">*No germinating spores detected in 4.6x10<sup>9</sup> spores.</p>
+
<p align="justify">*No germinating spores detected in 4.6x10<sup>9</sup> spores. For a full explanation of these great results, see [https://2012.igem.org/Team:LMU-Munich/Data/Knockout All results].</p>
-
===Inverter===
+
===Inverter works!===
-
 
+
<br>
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
| style="width: 70%;background-color: #EBFCE4;" |
| style="width: 70%;background-color: #EBFCE4;" |

Latest revision as of 22:13, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU red and white.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Results"