Team:LMU-Munich/Project

From 2012.igem.org

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== '''Overall project''' ==
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<html>
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[[File:Project draft.png|500px|center]]
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<style type="text/css">
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The goal of our project BEADzillus is the production of ''Bacillus subtilis'' spores which display different proteins with special features on their surface.
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#buttons {
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For example, the spore could:
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position:relative;
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}
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- bind harmful viruses
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#buttons img {
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display: block;
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position: absolute;
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top: 0;
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left: 0;
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z-index: -100;
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}
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- bind toxic metals
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#buttons a img {
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display: none;
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}
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- bind plastic molecules
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#buttons a:hover img {
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display: block;
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}
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- expose enzymes
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#buttons a {
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display: block;
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width: 50px;
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height: 50px;
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position: absolute;
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z-index 100;
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/* outline: 1px solid red; */
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}
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</style>
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<div id="buttons" style="height: 375px;">
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<img alt="" src="http://wrtlprnft.ath.cx/igem2012_home.png">
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<a href="https://2012.igem.org/Team:LMU-Munich/Project" style="left: 276px; top: 40px; width: 271px; height:265px;"><img style="left: -276px; top: -40px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home0.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins" style="left: 130px; top: 50px; width: 146px; height:145px;"><img style="left: -130px; top: -50px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home1.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop" style="left: 166px; top: 196px; width: 110px; height:110px;"><img style="left: -166px; top: -196px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home2.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks" style="left: 55px; top: 160px; width: 110px; height:110px;"><img style="left: -55px; top: -160px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home3.png"></a>
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</div>
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</html>
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These functions could be used to filter fluids or for use in laboratory.
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<p align=justify>
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Our project '''Bead'''zillus is composed of several modules. Click on the symbols to learn more about their function.
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As a safety consideration, we will stop spores from germinating. First, we want to knock out several genes which are important for germination from the spore phase; second, we want potentially germinating spores to be killed by a toxin which they produce themselves.
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The idea of '''Bead'''zillus is to enrich the [http://partsregistry.org/Main_Page partsregistry] with new BioBricks that are needed for the work with ''B. subtilis'' and show how its unique features lead to new creative ideas. We use the spores of ''B. subitlis'' to establish a platform for protein display.
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</p>
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*[[File:Bacilluss_Intro.png|30px|link=Team:LMU-Munich/Bacillus_Introduction]] [[Team:LMU-Munich/Bacillus_Introduction|'''''Bacillus'' Intro''']]: A short introduction to the organism ''Bacillus subtilis'' and why we chose to work with it.
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*[[File:BacillusBioBrickBox.png|30px|link=Team:LMU-Munich/Bacillus_BioBricks]] Our [[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]] offers a set of new parts for the work with ''B. subtilis''.
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*[[File:SporeCoat.png|30px|link=Team:LMU-Munich/Spore_Coat_Proteins]] [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]] are a platform to display proteins on ''B. subtilis'' spores.
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*[[File:GerminationSTOP.png|25px|link=Team:LMU-Munich/Germination_Stop]] [[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]] prevents the spores from germinating to vegetative cells.
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*On our [[Team:LMU-Munich/Data|Data]] page you find all our results in detail.
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*Why did we chose spores to display proteins and for what could they be used? See our [[Team:LMU-Munich/Application|Applications page]].
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Visit the other pages related to our project:
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* [[Team:LMU-Munich/Attributions|Attributions]] who helped us.
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* [[Team:LMU-Munich/Collaborations|Collaborations]] with other iGEM Teams.
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* [[Team:LMU-Munich/Parts|Registry]] Here is the list of all new BioBricks we've added to the registry so far.
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* [[Team:LMU-Munich/Strains|''Bacillus'' strains]] Here you find information about the ''B. subtilis'' strains we created and how to obtain them.
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* [[Team:LMU-Munich/Inverter|Inverter]] See here a special project we continued from the LMU-Munich 2011 Team.
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* [[Team:LMU-Munich/Achievements|Achievements]] Find here which medal we would give ourselves and why.
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'''Project Navigation'''
 
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{|
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|<font color="#FFFFFF">+--+--+--+--+--+--+--</font>
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<div class="box">
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|<font color="#FFFFFF">+--+--+--+--+--</font>
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====Project Navigation====
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|<font color="#FFFFFF">+--+--+--+--+--+--</font>
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{| width="100%" align="center" style="text-align:center;"
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|<font color="#FFFFFF">+--+--+--+--+--+--</font>
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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<html>
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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</a>
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<font size="1">
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</font>
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<br />
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction"><font size="1" face="verdana">Bacillus<BR>Intro</font></a>
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</html>
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|<html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
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<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=70"/>
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</a>
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<font size="1">
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</font>
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<br />
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
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</html>
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|<html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
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<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=70"/>
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</a>
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<font size="1">
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</font>
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<br />
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
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</html>
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|<html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
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<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=70"/>
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</a>
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<font size="1">
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</font>
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<br />
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
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</html>
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|}
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</div>
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 12:25, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU red and white.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

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