Team:LMU-Munich/Lab Notebook

From 2012.igem.org

(Difference between revisions)
Line 3: Line 3:
==Lab Notebook==
==Lab Notebook==
-
still in progress...
+
<html>
 +
 
 +
<head>
 +
<meta http-equiv=Content-Type content="text/html; charset=macintosh">
 +
<meta name=ProgId content=Excel.Sheet>
 +
<meta name=Generator content="Microsoft Excel 14">
 +
<link rel=File-List
 +
href="Weekly_Journal_Accomplishment_Worksheet-Dateien/filelist.xml">
 +
<style>
 +
<!--table
 +
{mso-displayed-decimal-separator:"\,";
 +
mso-displayed-thousand-separator:"\.";}
 +
@page
 +
{margin:.75in .7in .75in .7in;
 +
mso-header-margin:.51in;
 +
mso-footer-margin:.51in;}
 +
.font0
 +
{color:black;
 +
font-size:11.0pt;
 +
font-weight:400;
 +
font-style:normal;
 +
text-decoration:none;
 +
font-family:Calibri, sans-serif;
 +
mso-font-charset:0;}
 +
.font5
 +
{color:black;
 +
font-size:11.0pt;
 +
font-weight:700;
 +
font-style:normal;
 +
text-decoration:none;
 +
font-family:Calibri, sans-serif;
 +
mso-font-charset:0;}
 +
.font6
 +
{color:black;
 +
font-size:11.0pt;
 +
font-weight:400;
 +
font-style:italic;
 +
text-decoration:none;
 +
font-family:Calibri, sans-serif;
 +
mso-font-charset:0;}
 +
.style0
 +
{mso-number-format:General;
 +
text-align:general;
 +
vertical-align:bottom;
 +
white-space:nowrap;
 +
mso-rotate:0;
 +
mso-background-source:auto;
 +
mso-pattern:auto;
 +
color:black;
 +
font-size:11.0pt;
 +
font-weight:400;
 +
font-style:normal;
 +
text-decoration:none;
 +
font-family:Calibri, sans-serif;
 +
mso-font-charset:0;
 +
border:none;
 +
mso-protection:locked visible;
 +
mso-style-name:Standard;
 +
mso-style-id:0;}
 +
td
 +
{mso-style-parent:style0;
 +
padding-top:1px;
 +
padding-right:1px;
 +
padding-left:1px;
 +
mso-ignore:padding;
 +
color:black;
 +
font-size:11.0pt;
 +
font-weight:400;
 +
font-style:normal;
 +
text-decoration:none;
 +
font-family:Calibri, sans-serif;
 +
mso-font-charset:0;
 +
mso-number-format:General;
 +
text-align:general;
 +
vertical-align:bottom;
 +
border:none;
 +
mso-background-source:auto;
 +
mso-pattern:auto;
 +
mso-protection:locked visible;
 +
white-space:nowrap;
 +
mso-rotate:0;}
 +
.xl65
 +
{mso-style-parent:style0;
 +
font-weight:700;
 +
text-align:center;
 +
vertical-align:middle;
 +
border:1.0pt solid windowtext;}
 +
.xl66
 +
{mso-style-parent:style0;
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:none;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;
 +
white-space:normal;}
 +
.xl67
 +
{mso-style-parent:style0;
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:.5pt solid windowtext;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;
 +
white-space:normal;}
 +
.xl68
 +
{mso-style-parent:style0;
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:.5pt solid windowtext;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;}
 +
.xl69
 +
{mso-style-parent:style0;
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:.5pt solid windowtext;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:1.0pt solid windowtext;
 +
border-left:1.0pt solid windowtext;}
 +
.xl70
 +
{mso-style-parent:style0;
 +
font-weight:700;
 +
mso-number-format:"m\/d\/yyyy";
 +
text-align:center;
 +
vertical-align:middle;
 +
border:1.0pt solid windowtext;}
 +
.xl71
 +
{mso-style-parent:style0;
 +
mso-number-format:"m\/d\/yyyy";
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:none;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;
 +
white-space:normal;}
 +
.xl72
 +
{mso-style-parent:style0;
 +
mso-number-format:"m\/d\/yyyy";
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:.5pt solid windowtext;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;
 +
white-space:normal;}
 +
.xl73
 +
{mso-style-parent:style0;
 +
mso-number-format:"m\/d\/yyyy";
 +
text-align:center;
 +
vertical-align:middle;
 +
border-top:.5pt solid windowtext;
 +
border-right:1.0pt solid windowtext;
 +
border-bottom:.5pt solid windowtext;
 +
border-left:1.0pt solid windowtext;}
 +
-->
 +
</style>
 +
</head>
 +
 
 +
<body link=blue vlink=purple class=xl65535>
 +
 
 +
<table border=0 cellpadding=0 cellspacing=0 width=728 style='border-collapse:
 +
collapse;table-layout:fixed;width:728pt'>
 +
<col class=xl65535 width=426 style='mso-width-source:userset;mso-width-alt:
 +
18176;width:426pt'>
 +
<col class=xl65535 width=63 style='mso-width-source:userset;mso-width-alt:
 +
2688;width:63pt'>
 +
<col class=xl65535 width=34 style='mso-width-source:userset;mso-width-alt:
 +
1450;width:34pt'>
 +
<col class=xl65535 width=64 span=2 style='mso-width-source:userset;mso-width-alt:
 +
2730;width:64pt'>
 +
<col class=xl65535 width=77 style='mso-width-source:userset;mso-width-alt:
 +
3285;width:77pt'>
 +
<tr height=15 style='height:15.0pt'>
 +
  <td height=15 class=xl65 width=426 style='height:15.0pt;width:426pt'>Accomplishment</td>
 +
  <td class=xl70 width=63 style='border-left:none;width:63pt'>Date</td>
 +
  <td class=xl65 width=34 style='border-left:none;width:34pt'>Week</td>
 +
  <td class=xl65 width=64 style='border-left:none;width:64pt'>Project</td>
 +
  <td class=xl65 width=64 style='border-left:none;width:64pt'>Your name</td>
 +
  <td class=xl65 width=77 style='border-left:none;width:77pt'>Data? (Yes/No)</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl66 width=426 style='height:28.0pt;width:426pt'>E.g.
 +
  Created double-mutant using resistance-cassette KOs:<font class="font6"> cwlD</font><font
 +
  class="font0">::kan+</font><font class="font6">gerD</font><font class="font0">::cat</font></td>
 +
  <td class=xl71 width=63 style='border-left:none;width:63pt'>8.8.2012</td>
 +
  <td class=xl66 width=34 style='border-left:none;width:34pt'>32</td>
 +
  <td class=xl66 width=64 style='border-left:none;width:64pt'>Germination<font
 +
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
 +
  <td class=xl66 width=64 style='border-left:none;width:64pt'>Jenny &amp; Jara</td>
 +
  <td class=xl66 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>First Single mutant: sleB::MLS</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.2.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>14</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Germination<font
 +
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>Single mutant: cwlJ::spec</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.2.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>14</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Germination<font
 +
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>Single mutant: gerD::cat</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.4.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>14</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Germination<font
 +
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>First Bacillus iGEM backbone! pSBBS0K-Pspac (from: pDG148-RFP)</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.5.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>14</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>Single mutant cwlD::kann</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.10.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>15</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Germination<font
 +
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=14 style='height:14.0pt'>
 +
  <td height=14 class=xl67 width=426 style='height:14.0pt;border-top:none;
 +
  width:426pt'>GFP-spore plasmids from Japan arrived</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.13.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>15</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Sporo<font
 +
  class="font5">beads</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>&nbsp;</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>Bacillus pSBBS (pAH328-RFP)</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>&nbsp;</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>0</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>pSBBS1C-lacZ (pAC6-RFP)</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>4.19.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>16</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr class=xl65535 height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>pSBBS4S-Pxyl (pXT-RFP)</td>
 +
  <td class=xl72 width=63 style='border-top:none;border-left:none;width:63pt'>7.9.2012</td>
 +
  <td class=xl67 width=34 style='border-top:none;border-left:none;width:34pt'>28</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Jara</td>
 +
  <td class=xl67 width=77 style='border-top:none;border-left:none;width:77pt'>&nbsp;</td>
 +
</tr>
 +
<tr height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl67 width=426 style='height:28.0pt;border-top:none;
 +
  width:426pt'>First empty Bacillus backbone pSBBS1C (pDG1662-RFP)</td>
 +
  <td class=xl73 style='border-top:none;border-left:none'>7.9.2012</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>28</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>Jara</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>&nbsp;</td>
 +
</tr>
 +
<tr height=28 style='height:28.0pt'>
 +
  <td height=28 class=xl68 style='height:28.0pt;border-top:none'>pSBBS4S
 +
  (pDG1731-RFP)</td>
 +
  <td class=xl73 style='border-top:none;border-left:none'>7.9.2012</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>28</td>
 +
  <td class=xl67 width=64 style='border-top:none;border-left:none;width:64pt'>Bacillus
 +
  BioBrick<font class="font5">Box</font></td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>Jara</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>&nbsp;</td>
 +
</tr>
 +
<tr height=15 style='height:15.0pt'>
 +
  <td height=15 class=xl69 style='height:15.0pt;border-top:none'>Double
 +
  negative loop with lacZa finished (works qualitativly</td>
 +
  <td class=xl73 style='border-top:none;border-left:none'>8.22.2012</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>34</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>double negative loop</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>Julia</td>
 +
  <td class=xl68 style='border-top:none;border-left:none'>&nbsp;</td>
 +
</tr>
 +
</table>
 +
 
 +
</body>
 +
 
 +
</html>
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Revision as of 12:04, 23 August 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo8.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde