Team:LMU-Munich/Germination Stop

From 2012.igem.org

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==What Methods Did We Use to Knockout Germination Genes?==
==What Methods Did We Use to Knockout Germination Genes?==
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<p align="justify">Two methods were employed to knock out germination: resistance cassette knockouts and clean deletions. Resistance cassette (RC) knockouts were performed using long-flanking-homology PCR (see Fig. 3 and [https://2012.igem.org/Team:LMU-Munich/Lab_Notebook/Protocols Protocols]). Single RC knockouts were created first; then they were combined to create multiple knockouts. The genome with all RC knockouts can be seen in Fig 4.</p>
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<p align="justify">Two methods were employed to knock out germination genes: replacement by resistance cassettes and clean deletions. Resistance cassette (RC) knockouts were performed using long-flanking homology PCR (see Fig. 3 and [https://2012.igem.org/Team:LMU-Munich/Lab_Notebook/Protocols Protocols]). Single RC knockouts were created first; then they were combined to create multiple knockouts. A graphical representation of the genome with all replacements with resistance cassettes can be seen in Fig 4.</p>
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Revision as of 16:08, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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