Team:LMU-Munich/Germination Stop

From 2012.igem.org

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<p align="justify">The germination rate of our mutants were checked with a [[Team:LMU-Munich/Lab_Notebook/Protocols|germination assay]]. The assay was developed based on the protocols of other researchers.
<p align="justify">The germination rate of our mutants were checked with a [[Team:LMU-Munich/Lab_Notebook/Protocols|germination assay]]. The assay was developed based on the protocols of other researchers.
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<p align="justify">We were able to achieve germination rates as low as '''<1 in 4.6x10<sup>9</sup>''' spores in our triple mutant B43. Other mutants also showed extremely reduced germination ability. A table of germination rates of our mutants and all other results can be found on [https://2012.igem.org/Team:LMU-Munich/Data/Knockout our results page].  
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<p align="justify">We were able to achieve germination rates as low as '''<1 in 4.6x10<sup>9</sup>''' spores in our triple mutant B43. Other mutants also showed extremely reduced germination ability. A table of germination rates of our mutants and all other results can be found on [https://2012.igem.org/Team:LMU-Munich/Data/Knockout our results page]. Our single mutants were not checked in the germination assay. The double and triple mutants tested showed mixed results, with some unable to germinate, and others maintaining germination ability. Our quadruple mutants demonstrated no ability to germinate, even several separate germination assays.
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<p align="justify">Despite this success, safety was a top priority for this project. In the event that some small percentage of spores retained the ability to germinate, the '''Suicide''' switch subproject (below) prevents outgrowth into viable vegetative cells.</p>
<p align="justify">Despite this success, safety was a top priority for this project. In the event that some small percentage of spores retained the ability to germinate, the '''Suicide''' switch subproject (below) prevents outgrowth into viable vegetative cells.</p>

Revision as of 17:50, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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