Team:LMU-Munich/Data/gfp spore

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<font color="#000000"; size="2">Fig. 1: Data of the various''' Sporo'''bead constructs</font>
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<p align="justify">Fig. 1 verifies the previously discribed diffuse background fluorescence of wild type spores (W168). The B49 strain even shows an increased fluorescence background. Nevertheless there is a signficant difference between gfp-'''Sporo'''beads and W168 and B 49,respectively. '''Sporo'''beads with present native ''cotZ'' revealed uniformal distribution around the edge, whereas the ones with the deletion of ''cotZ'' elucidated a clear "polar" distribution. Additionally there is a difference in fluorescence activity between the two versions of ''cotZ'' recognizable. It appears the strain B 70 with the construct [http://partsregistry.org/Part:BBa_K823049 P<sub>''cotYZ''</sub>-''cotZ''<sub>-2aa</sub>-''gfp''-''terminator''] and the deleted native ''cotZ'' had the strongest fluorescence. But as it does not have a uniformal fusion protein distribution, B 53 would be the strain for future '''Sporo'''bead applications.  
<p align="justify">Fig. 1 verifies the previously discribed diffuse background fluorescence of wild type spores (W168). The B49 strain even shows an increased fluorescence background. Nevertheless there is a signficant difference between gfp-'''Sporo'''beads and W168 and B 49,respectively. '''Sporo'''beads with present native ''cotZ'' revealed uniformal distribution around the edge, whereas the ones with the deletion of ''cotZ'' elucidated a clear "polar" distribution. Additionally there is a difference in fluorescence activity between the two versions of ''cotZ'' recognizable. It appears the strain B 70 with the construct [http://partsregistry.org/Part:BBa_K823049 P<sub>''cotYZ''</sub>-''cotZ''<sub>-2aa</sub>-''gfp''-''terminator''] and the deleted native ''cotZ'' had the strongest fluorescence. But as it does not have a uniformal fusion protein distribution, B 53 would be the strain for future '''Sporo'''bead applications.  

Revision as of 02:05, 27 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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