Team:LMU-Munich/Data/crustpromoters

From 2012.igem.org

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====Crust promoter measurements====
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====Crust promoter evaluation====
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<p align="justify">The figure below shows the result of the evaluation of the three promoters for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. Each promoter (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) has two independent clones and both were measured three times. To be able to know in which growth phase the cells are the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
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<p align="justify">The figure below shows the result of the evaluation of the three promoters (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. For each promoter two independent clones were measured three times.To link the promoter activities to the growth phase, the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
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{| style="color:black;" cellpadding="0" width="90%" cellspacing="0" border="0" align="center" style="text-align:left;"
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<font color="#000000"; size="2"><p align="justify">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub>. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
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<font color="#000000"; size="2"><p align="justify">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub> fused to the lux operon. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
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<p align="justify">The native promoter of ''cotZ'' P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub> show a signal in stationary phase whereas P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub>. It is displayed that these promoters are active for about 12 hours while  P<sub>''cgeA''</sub> does not show any acitvity. </p>
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<p align="justify">The native promoter of ''cotZ'', P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub>, show a signal in stationary phase. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. P<sub>''cgeA''</sub> does not show any acitvity. </p>

Revision as of 20:48, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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