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Team:LMU-Munich/Data/Vectors
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pSB<sub>Bs</sub>1C is one of our empty vectors. It integrates into the ''amy''E locus of ''Bacillus subtilis'' and carries a Chloramphenicol resistance as well as an Ampicillin resistance for ''E. coli''. Inbetween the two recombination sites, there is the constitutively expressed Cm resistance and the multiple cloning site which contains RFP to simplify the insertion of your BioBrick. | pSB<sub>Bs</sub>1C is one of our empty vectors. It integrates into the ''amy''E locus of ''Bacillus subtilis'' and carries a Chloramphenicol resistance as well as an Ampicillin resistance for ''E. coli''. Inbetween the two recombination sites, there is the constitutively expressed Cm resistance and the multiple cloning site which contains RFP to simplify the insertion of your BioBrick. | ||
| + | [File:LMU-Munich-starchplate.JPG | 300px|right] | ||
The plasmid is then transformed into ''B. subtilis'' and checked via the resistance for "integration at all" and via a starch test for "integration into the right locus". | The plasmid is then transformed into ''B. subtilis'' and checked via the resistance for "integration at all" and via a starch test for "integration into the right locus". | ||
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We tested this vector with various inserts, for example the final construct of our Sporobeads with GFP. The starch test shows that the insertion in in the ''amy''E locus and fluorescence microscopy reveals the functionality of the construct. | We tested this vector with various inserts, for example the final construct of our Sporobeads with GFP. The starch test shows that the insertion in in the ''amy''E locus and fluorescence microscopy reveals the functionality of the construct. | ||
Revision as of 14:46, 21 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Bacillus subtilis vectors
For the detailed protocols, please see our protocol section
pSBBs1C
pSBBs1C is one of our empty vectors. It integrates into the amyE locus of Bacillus subtilis and carries a Chloramphenicol resistance as well as an Ampicillin resistance for E. coli. Inbetween the two recombination sites, there is the constitutively expressed Cm resistance and the multiple cloning site which contains RFP to simplify the insertion of your BioBrick.
[File:LMU-Munich-starchplate.JPG | 300px|right]
The plasmid is then transformed into B. subtilis and checked via the resistance for "integration at all" and via a starch test for "integration into the right locus".
We tested this vector with various inserts, for example the final construct of our Sporobeads with GFP. The starch test shows that the insertion in in the amyE locus and fluorescence microscopy reveals the functionality of the construct.
pSBBs4S
pSBBs2E
pSBBs0K-Pspac
pSBBs4S-PXyl
pSBBs1C-lacZ
pSBBs3C-luxABCDE
