Team:LMU-Munich/Data/Knockout

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<font color="#000000"; size="2">Fig 1: '''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. W168 showed near 100% germination rates. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Our results were consistent with this expectation. Mutants used were: <br />
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<font color="#000000"; size="2"><p align="justify">Fig 1: '''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. W168 showed near 100% germination rates. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Our results were consistent with this expectation. Mutants used were:</p> <br />
'''W168''': wild-type 168 <br />
'''W168''': wild-type 168 <br />
'''''spo0A''::tet''': a sporulation-deficient strain <br />
'''''spo0A''::tet''': a sporulation-deficient strain <br />
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<font color="#000000"; size="2">Fig. 2: '''Plates of germination mutants.''' Plates contained heated (to kill vegetative cells and leave only spores to germinate) cultures and unheated (where colonies may come from germinated spores and the vegetative cells) cultures. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). The mistake of photographing empty plates against a background of spots was realized after plates had been discarded. Presence of colonies is noted below, for clarity. Mutants used were: <br />
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<font color="#000000"; size="2"><p align="justify">Fig. 2: '''Plates of germination mutants.''' Plates contained heated (to kill vegetative cells and leave only spores to germinate) cultures and unheated (where colonies may come from germinated spores and the vegetative cells) cultures. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). The mistake of photographing empty plates against a background of spots was realized after plates had been discarded. Presence of colonies is noted below, for clarity. Mutants used were:</p> <br />
'''W168''': Wild-type 168; <span style="color:red">Heated plate: colonies present</span>; Unheated plate: colonies present <br />
'''W168''': Wild-type 168; <span style="color:red">Heated plate: colonies present</span>; Unheated plate: colonies present <br />
'''''spo0A''::tet''': A sporulation-deficient strain; <span style="color:red">Heated plate: no colonies</span>; Unheated plate: colonies present <br />
'''''spo0A''::tet''': A sporulation-deficient strain; <span style="color:red">Heated plate: no colonies</span>; Unheated plate: colonies present <br />
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Table 1: Germination rates of our mutant strains.
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<font color="#000000"; size="2">'''Germinable spores per mutant.'''</font>
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Latest revision as of 01:56, 27 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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