Team:LMU-Munich/Data/Inverter

From 2012.igem.org

(Difference between revisions)
Line 6: Line 6:
==== β-galoctisidase assay of the Inverter with ''lacZα''====
==== β-galoctisidase assay of the Inverter with ''lacZα''====
-
[[File:LMU Inverter graph.png|600px]]
+
 
 +
{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{|
 +
|[[File:LMU Inverter graph.png|600px|center]]
 +
|-
 +
| style="width: 70%;background-color: #EBFCE4;" |
 +
{| style="color:black;" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
 +
|style="width: 70%;background-color: #EBFCE4;" |
 +
<font color="#000000"; size="2"><p align="justify"> β-Galactosidase assay of Inverter with ''lacZα'' as reporter. The higher the arabinose concentration, the higher the translational repression of ''uof<sub>CGU</sub>-lacZα'' </p></font>
 +
|}
 +
|}
 +
|}
The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uof<sub>CGU</sub> and masks the Shine Dalgarno sequence, thereby translationally repressing it. Uof<sub>CGU</sub> is translationally fused to ''lacZα'', and therefore also translationally repressed. Uof<sub>CGU</sub>-''lacZα'' is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The P<sub>''BAD''</sub> is titrated with Arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the ''lacZα'' translational repression.
The Inverter is composed of the pBAD promoter (lacking the repressor binding sites), which produces the short RNA RyhB. RyhB binds to uof<sub>CGU</sub> and masks the Shine Dalgarno sequence, thereby translationally repressing it. Uof<sub>CGU</sub> is translationally fused to ''lacZα'', and therefore also translationally repressed. Uof<sub>CGU</sub>-''lacZα'' is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is induced with 1mM IPTG. The P<sub>''BAD''</sub> is titrated with Arabinose. The higher the arabinose concentration is, the higher the RyhB concentration and consequently the ''lacZα'' translational repression.

Revision as of 17:29, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo9.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde