Team:LMU-Munich/Data/Constitutive

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The constitutive promoters '''P<sub>''liaG''</sub>''' and '''P<sub>''lepA''</sub>''' were evaluated in the reporter vector pSB<sub>Bs</sub>3C-<i>luxABCDE</i>, which contains the ''lux'' operon [[File:Lux operon.png|100px]]. The promoter activity results in gene expression and production of the protein luciferase. The luminescence produced by this protein can be measured with the plate reader ''Synergy2'' ([http://www.biotek.com/ BioTek]) ('''Fig.1'''). </p>
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The constitutive promoters '''P<sub>''liaG''</sub>''' and '''P<sub>''lepA''</sub>''' were evaluated in the reporter vector pSB<sub>Bs</sub>3C-<i>luxABCDE</i>, which contains the ''lux'' operon [[File:Lux operon.png|100px]]. The promoter activity results in gene expression and production of the protein luciferase. The luminescence produced by this protein can be measured with the plate reader ''Synergy2'' ([http://www.biotek.com/ BioTek]) (Fig. 1). </p>
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===β-galactosidase assays===
===β-galactosidase assays===
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<p align="justify"> The two constitutive promoters '''P<sub>''liaG''</sub>''' and '''P<sub>''veg''</sub>''' were evaluated with the reporter vector pSB<sub>''Bs''</sub>1C-''lacZ'', which contains the ''lacZ'' reporter gene [[File:LacZ.png|50px]] ('''Fig.2''').</p>
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<p align="justify"> The two constitutive promoters '''P<sub>''liaG''</sub>''' and '''P<sub>''veg''</sub>''' were evaluated with the reporter vector pSB<sub>''Bs''</sub>1C-''lacZ'', which contains the ''lacZ'' reporter gene [[File:LacZ.png|50px]] (Fig. 2).</p>
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Latest revision as of 13:23, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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