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Team:LMU-Munich/Data
From 2012.igem.org
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====Constitutive ''Bacillus'' Promoters==== | ====Constitutive ''Bacillus'' Promoters==== | ||
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| - | |<p align="justify">Evaluation of constitutive ''B. subtilis'' promoters with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p> | + | |<p align="justify">Evaluation of the constitutive ''B. subtilis'' promoters P<sub>''liaG''</sub>, P<sub>''veg''</sub> and P<sub>''lepA''</sub> with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p> |
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]] | |[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]] | ||
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====Inducible ''Bacillus'' Promoters==== | ====Inducible ''Bacillus'' Promoters==== | ||
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| - | |<p align="justify">Evaluation of inducible ''B. subtilis'' | + | |<p align="justify">Evaluation of the inducible ''B. subtilis'' promoter P<sub>''liaI''</sub> with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p> |
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]] | |[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]] | ||
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Revision as of 18:22, 23 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Data
Here you will find all of our project data.
BacillusBioBrickBox
Evaluation of B. subtilis vectors
Evaluation of B. subtilis vectors in B. subtilis with different inserts. |  |
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Anderson Promoter Evaluation
Evaluation of the Anderson promoters in B. subtilis with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Constitutive Bacillus Promoters
Evaluation of the constitutive B. subtilis promoters PliaG, Pveg and PlepA with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Inducible Bacillus Promoters
Evaluation of the inducible B. subtilis promoter PliaI with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays). | 
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Sporobeads
Spore purification
We compared different ways of purifying Bacillus spores from vegetative cells | 
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GerminationSTOP
Knockouts of germination genes
Germination assy of Bacillus strains with multiple gene knockouts | 
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Trashcan
Things that just don't want to work out
cgeA and PcgeA
Unexpected restriction sites combined with no activity at all |
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MazF
The story of trying to clone a toxine from E. coli |
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For our big breakthroughs, or to follow specific projects, see the individual project pages:
